| Title |
A lateral flow colloidal gold-based immunoassay for rapid detection of miroestrol in samples of White Kwao Krua, a phytoestrogen-rich plant
|
|---|---|
| Published in |
Journal of Natural Medicines, June 2017
|
| DOI | 10.1007/s11418-017-1096-3 |
| Pubmed ID | |
| Authors |
Tharita Kitisripanya, Chadathorn Inyai, Jukrapun Komaikul, Supaluk Krittanai, Thaweesak Juengwatanatrakul, Seiichi Sakamoto, Hiroyuki Tanaka, Satoshi Morimoto, Waraporn Putalun |
| Abstract |
White Kwao Krua (WKK)-derived products have been used worldwide as dietary supplements to relieve climacteric symptoms in menopausal women. Miroestrol is a unique chromene found in WKK tuberous roots that corresponds to the estrogenic activity of WKK. However, miroestrol naturally accumulates at low levels in WKK samples, which are difficult to detect. The development of a rapid and sensitive assay to detect miroestrol in numerous products derived from this plant would be a practical and useful method to guarantee the quality of raw materials. To allow rapid and easy qualitative detection of miroestrol, a lateral flow immunoassay (LFIA) using a colloidal gold-labeled monoclonal antibody (mAb) against miroestrol was developed. The qualitative LFIA was based on the competition of free miroestrol in the sample and immobilized miroestrol-conjugated proteins on the strip for a limited number of antibodies in the detection reagent. Anti-miroestrol mAb was colored by colloidal gold labels and used as the detection reagent in LFIA. Anti-mouse immunoglobulin G was used to indicate the functioning of the LFIA system. The detection limit of the LFIA was 0.156 μg of miroestrol. The LFIA was applied to determine the miroestrol content in WKK samples and products. The result was compared with the validated enzyme-linked immunosorbent assay (ELISA) and demonstrated a correlative outcome. This study shows that the developed LFIA is practical and suitable for detecting small amounts of miroestrol in WKK samples. This qualitative assay is more rapid in screening miroestrol in WKK samples (within 10 min) than conventional methods (ELISA and HPLC). |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 24% |
| Researcher | 2 | 12% |
| Student > Ph. D. Student | 2 | 12% |
| Student > Bachelor | 1 | 6% |
| Professor | 1 | 6% |
| Other | 3 | 18% |
| Unknown | 4 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 24% |
| Engineering | 2 | 12% |
| Environmental Science | 1 | 6% |
| Chemical Engineering | 1 | 6% |
| Arts and Humanities | 1 | 6% |
| Other | 4 | 24% |
| Unknown | 4 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 June 2017.
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#20,425,762
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Outputs from Journal of Natural Medicines
#397
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#275,569
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Outputs of similar age from Journal of Natural Medicines
#9
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