| Title |
Microbial synthesis of gold nanoparticles using the fungus Penicillium brevicompactum and their cytotoxic effects against mouse mayo blast cancer C2C12 cells
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, September 2011
|
| DOI | 10.1007/s00253-011-3556-0 |
| Pubmed ID | |
| Authors |
Amrita Mishra, Suraj Kumar Tripathy, Rizwan Wahab, Song-Hoon Jeong, Inho Hwang, You-Bing Yang, Young-Soon Kim, Hyung-Shik Shin, Soon-Il Yun |
| Abstract |
Microorganisms, their cell filtrates, and live biomass have been utilized for synthesizing various gold nanoparticles. The shape, size, stability as well as the purity of the bio synthesized nanoparticles become very essential for application purpose. In the present study, gold nanoparticles have been synthesized from the supernatant, live cell filtrate, and biomass of the fungus Penicillium brevicompactum. The fungus has been grown in potato dextrose broth which is also found to synthesize gold nanoparticles. The size of the particles has been investigated by Bio-TEM before purification, following purification and after storing the particles for 3 months under refrigerated condition. Different characterization techniques like X-ray diffraction, Fourier transform infrared spectroscopy, and UV-visible spectroscopy have been used for analysis of the particles. The effect of reaction parameters such as pH and concentration of gold salt have also been monitored to optimize the morphology and dispersity of the synthesized gold nanoparticles. A pH range of 5 to 8 has favored the synthesis process whereas increasing concentration of gold salt (beyond 2 mM) has resulted in the formation of bigger sized and aggregated nanoparticles. Additionally, the cytotoxic nature of prepared nanoparticles has been analyzed using mouse mayo blast cancer C(2)C(12) cells at different time intervals (24, 48, and 72 h) of incubation period. The cells are cultivated in Dulbecco's modified Eagle's medium supplemented with fetal bovine serum with antibiotics (streptopenicillin) at 37°C in a 5% humidified environment of CO(2). The medium has been replenished every other day, and the cells are subcultured after reaching the confluence. The viability of the cells is analyzed with 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide method. |
Mendeley readers
The data shown below were compiled from readership statistics for 124 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | <1% |
| India | 1 | <1% |
| Russia | 1 | <1% |
| Brazil | 1 | <1% |
| Unknown | 120 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 25 | 20% |
| Researcher | 15 | 12% |
| Student > Master | 15 | 12% |
| Student > Bachelor | 12 | 10% |
| Professor > Associate Professor | 6 | 5% |
| Other | 18 | 15% |
| Unknown | 33 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 26 | 21% |
| Chemistry | 12 | 10% |
| Biochemistry, Genetics and Molecular Biology | 11 | 9% |
| Chemical Engineering | 6 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 6 | 5% |
| Other | 19 | 15% |
| Unknown | 44 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 December 2011.
All research outputs
#6,324,643
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#2,282
of 8,034 outputs
Outputs of similar age
#35,120
of 128,526 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#20
of 63 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one has gotten more attention than average, scoring higher than 71% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 128,526 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.
We're also able to compare this research output to 63 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.