| Title |
Bronchial microbiome of severe COPD patients colonised by Pseudomonas aeruginosa
|
|---|---|
| Published in |
European Journal of Clinical Microbiology & Infectious Diseases, January 2014
|
| DOI | 10.1007/s10096-013-2044-0 |
| Pubmed ID | |
| Authors |
L. Millares, R. Ferrari, M. Gallego, M. Garcia-Nuñez, V. Pérez-Brocal, M. Espasa, X. Pomares, C. Monton, A. Moya, E. Monsó |
| Abstract |
The bronchial microbiome in severe COPD during stability and exacerbation in patients chronically colonised by Pseudomonas aeruginosa (PA), has not been defined. Our objective was to determine the characteristics of the bronchial microbiome of severe COPD patients colonised and not colonised by P. aeruginosa and its changes during exacerbation. COPD patients with severe disease and frequent exacerbations were categorised according to chronic colonisation by P. aeruginosa. Sputum samples were obtained in stability and exacerbation, cultured, and analysed by 16S rRNA gene amplification and pyrosequencing. Sixteen patients were included, 5 of them showing chronic colonisation by P. aeruginosa. Pseudomonas genus had significantly higher relative abundance in stable colonised patients (p = 0.019), but no significant differences in biodiversity parameters were found between the two groups (Shannon, 3 (2-4) vs 3 (2-3), p = 0.699; Chao1, 124 (77-159) vs 140 (115-163), p = 0.364). In PA-colonised patients bronchial microbiome changed to a microbiome similar to non-PA-colonised patients during exacerbations. An increase in the relative abundance over 20 % during exacerbation was found for Streptococcus, Pseudomonas, Moraxella, Haemophilus, Neisseria, Achromobacter and Corynebacterium genera, which include recognised potentially pathogenic microorganisms, in 13 patients colonised and not colonised by P. aeruginosa with paired samples. These increases were not identified by culture in 5 out of 13 participants (38.5 %). Stable COPD patients with severe disease and PA-colonised showed a similar biodiversity to non-PA-colonised patients, with a higher relative abundance of Pseudomonas genus in bronchial secretions. Exacerbation in severe COPD patients showed the same microbial pattern, independently of previous colonisation by P. aeruginosa. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 139 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 2 | 1% |
| Korea, Republic of | 1 | <1% |
| Spain | 1 | <1% |
| United Kingdom | 1 | <1% |
| Unknown | 134 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 28 | 20% |
| Student > Ph. D. Student | 25 | 18% |
| Student > Master | 13 | 9% |
| Student > Bachelor | 11 | 8% |
| Other | 9 | 6% |
| Other | 25 | 18% |
| Unknown | 28 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 41 | 29% |
| Agricultural and Biological Sciences | 20 | 14% |
| Biochemistry, Genetics and Molecular Biology | 17 | 12% |
| Immunology and Microbiology | 15 | 11% |
| Nursing and Health Professions | 2 | 1% |
| Other | 8 | 6% |
| Unknown | 36 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 April 2014.
All research outputs
#12,892,336
of 22,743,667 outputs
Outputs from European Journal of Clinical Microbiology & Infectious Diseases
#1,542
of 2,769 outputs
Outputs of similar age
#155,139
of 305,702 outputs
Outputs of similar age from European Journal of Clinical Microbiology & Infectious Diseases
#22
of 40 outputs
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We're also able to compare this research output to 40 others from the same source and published within six weeks on either side of this one. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.