| Title |
Antibacterial and anticancer activities of acetone extracts from in vitro cultured lichen-forming fungi
|
|---|---|
| Published in |
BMC Complementary Medicine and Therapies, June 2017
|
| DOI | 10.1186/s12906-017-1819-8 |
| Pubmed ID | |
| Authors |
Agnieszka Felczykowska, Alicja Pastuszak-Skrzypczak, Anna Pawlik, Krystyna Bogucka, Anna Herman-Antosiewicz, Beata Guzow-Krzemińska |
| Abstract |
Lichens that were used in traditional medicine for ages produce numerous secondary metabolites, however our knowledge about biological activities of substances secreted by separated bionts is scarce. The main objectives of this study were to isolate and find optimal conditions for the growth of mycelia from three common lichen-forming fungi, i.e. Caloplaca pusilla, Protoparmeliopsis muralis and Xanthoria parietina and to evaluate antibacterial and antiproliferative activities of their acetone extracts. Agar disc diffusion and broth microdilution methods were used to test antimicrobial activity against six species of bacteria. MTT method, flow cytometry assay and DAPI staining were applied to test antiproliferative activity of selected extracts against MCF-7 (human breast adenocarcinoma), PC-3 (human prostate cancer) and HeLa (human cervix adenocarcinoma) cancer cells. P. muralis strongly inhibited the growth of Gram-positive bacteria, i.e. Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus and Staphylococcus epidermidis (MICs from 6.67 to 100.00 μg mL(-1)). X. parietina grown on PDA and G-LBM media decreased HeLa or MCF-7 cancer cells viability with IC50 values of about 8 μg mL(-1), while C. pusilla grown on G-LBM medium showed the highest potency in decreasing MCF-7 (7.29 μg mL(-1)), PC-3 (7.96 μg mL(-1)) and HeLa (6.57 μg mL(-1)) cancer cells viability. We also showed induction of apoptosis in HeLa, PC-3 and MCF-7 cell lines treated with increasing concentrations of C. pusilla extract. We showed that selected acetone extracts demonstrated a strong antimicrobial and anticancer effects that suggests that aposymbiotically cultured lichen-forming fungi can be a source of antibacterial and antiproliferative compounds. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 52 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 23% |
| Student > Bachelor | 6 | 12% |
| Student > Master | 6 | 12% |
| Researcher | 5 | 10% |
| Student > Doctoral Student | 2 | 4% |
| Other | 3 | 6% |
| Unknown | 18 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 19% |
| Agricultural and Biological Sciences | 9 | 17% |
| Pharmacology, Toxicology and Pharmaceutical Science | 6 | 12% |
| Immunology and Microbiology | 3 | 6% |
| Chemistry | 2 | 4% |
| Other | 3 | 6% |
| Unknown | 19 | 37% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 June 2017.
All research outputs
#17,898,929
of 22,979,862 outputs
Outputs from BMC Complementary Medicine and Therapies
#2,359
of 3,641 outputs
Outputs of similar age
#226,959
of 317,348 outputs
Outputs of similar age from BMC Complementary Medicine and Therapies
#77
of 133 outputs
Altmetric has tracked 22,979,862 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,641 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.6. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
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