| Title |
Development of a quantitative Correlative Light Electron Microscopy technique to study GLUT4 trafficking
|
|---|---|
| Published in |
Protoplasma, January 2014
|
| DOI | 10.1007/s00709-013-0597-5 |
| Pubmed ID | |
| Authors |
Lorna Hodgson, Jeremy Tavaré, Paul Verkade |
| Abstract |
Correlative Light Electron Microscopy (CLEM) combines advantages of light microscopy and electron microscopy in one experiment to deliver information above and beyond the capability of either modality alone. There are many different CLEM techniques, each having its own special advantages but also its technical challenges. It is however the biological question that (should) drive(s) the development and application of a specific CLEM technique in order to provide the answer. Here we describe the development of a CLEM technique that is based on the Tokuyasu cryo immuno-gold labelling technique that has allowed us to quantitatively study GLUT4 trafficking. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 2% |
| Netherlands | 1 | 2% |
| United States | 1 | 2% |
| Germany | 1 | 2% |
| Unknown | 56 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 22 | 37% |
| Researcher | 15 | 25% |
| Student > Doctoral Student | 5 | 8% |
| Other | 4 | 7% |
| Student > Bachelor | 2 | 3% |
| Other | 8 | 13% |
| Unknown | 4 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 23 | 38% |
| Biochemistry, Genetics and Molecular Biology | 13 | 22% |
| Chemistry | 6 | 10% |
| Medicine and Dentistry | 4 | 7% |
| Physics and Astronomy | 3 | 5% |
| Other | 9 | 15% |
| Unknown | 2 | 3% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 February 2014.
All research outputs
#13,054,270
of 22,743,667 outputs
Outputs from Protoplasma
#384
of 968 outputs
Outputs of similar age
#158,759
of 304,897 outputs
Outputs of similar age from Protoplasma
#6
of 13 outputs
Altmetric has tracked 22,743,667 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 968 research outputs from this source. They receive a mean Attention Score of 2.4. This one has gotten more attention than average, scoring higher than 60% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 304,897 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.