| Title |
Design of riboregulators for control of cyanobacterial (Synechocystis) protein expression
|
|---|---|
| Published in |
Biotechnology Techniques, September 2013
|
| DOI | 10.1007/s10529-013-1352-x |
| Pubmed ID | |
| Authors |
Koichi Abe, Yuta Sakai, Saki Nakashima, Masataka Araki, Wataru Yoshida, Koji Sode, Kazunori Ikebukuro |
| Abstract |
Cyanobacteria are attractive host bacteria for biofuel production because they can covert CO2 to biofuel lipids using only sunlight, water, and inorganic ions. For genetically engineering an ideal cyanobacterium, a synthetic biological approach is promising but few genetic components have been characterized in cyanobacteria. Here for controlling cyanobacterial protein expression, we constructed riboregulators, that one of the post-transcriptional regulators composed of RNAs. Riboregulators harboring a ribosome-binding site suitable for Synechocystis sp. were designed by trial and error using Escherichia coli as host bacteria. The designed riboregulators were effective in Synechocystis sp. as well as E. coli with slight interference on growth only observed in E. coli. They will therefore be useful tools for controlling target gene expression. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 68 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 3% |
| United States | 1 | 1% |
| Netherlands | 1 | 1% |
| Unknown | 64 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 14 | 21% |
| Researcher | 13 | 19% |
| Student > Master | 8 | 12% |
| Student > Bachelor | 7 | 10% |
| Professor | 5 | 7% |
| Other | 10 | 15% |
| Unknown | 11 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 26 | 38% |
| Biochemistry, Genetics and Molecular Biology | 20 | 29% |
| Engineering | 3 | 4% |
| Chemical Engineering | 2 | 3% |
| Environmental Science | 1 | 1% |
| Other | 4 | 6% |
| Unknown | 12 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 February 2014.
All research outputs
#17,286,379
of 25,374,647 outputs
Outputs from Biotechnology Techniques
#2,205
of 2,762 outputs
Outputs of similar age
#134,747
of 215,410 outputs
Outputs of similar age from Biotechnology Techniques
#20
of 30 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,762 research outputs from this source. They receive a mean Attention Score of 3.9. This one is in the 16th percentile – i.e., 16% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 215,410 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 30 others from the same source and published within six weeks on either side of this one. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.