| Title |
Two variants of recombinant human bone morphogenetic protein-2 (rhBMP-2) with additional protein domains: Synthesis in an Escherichia coli heterologous expression system
|
|---|---|
| Published in |
Biochemistry, May 2017
|
| DOI | 10.1134/s0006297917050091 |
| Pubmed ID | |
| Authors |
A. S. Karyagina, I. S. Boksha, T. M. Grunina, A. V. Demidenko, M. S. Poponova, O. V. Sergienko, A. M. Lyashchuk, Z. M. Galushkina, L. A. Soboleva, E. O. Osidak, M. S. Bartov, A. V. Gromov, V. G. Lunin |
| Abstract |
Two variants of recombinant human bone morphogenetic protein-2 (rhBMP-2) with additional N-terminal protein domains were obtained by expression in E. coli. The N-terminal domains were s-tag (15-a.a. oligopeptide from bovine pancreatic ribonuclease A) and lz (leucine zipper dimerization domain from yeast transcription factor GCN4). The s-tag-BMP-2 and lz-BMP-2 were purified by a procedure that excluded a long refolding stage. The resulting dimeric proteins displayed higher solubility compared to rhBMP-2 without additional protein domains. Biological activity of both proteins was demonstrated in vitro by induction of alkaline phosphatase in C2C12 cells, and the activity of s-tag-BMP-2 in vivo was shown in various experimental animal models. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 19% |
| Student > Bachelor | 2 | 13% |
| Student > Master | 2 | 13% |
| Student > Ph. D. Student | 2 | 13% |
| Other | 1 | 6% |
| Other | 2 | 13% |
| Unknown | 4 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 19% |
| Chemistry | 2 | 13% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 13% |
| Chemical Engineering | 1 | 6% |
| Medicine and Dentistry | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 6 | 38% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 June 2017.
All research outputs
#22,834,739
of 25,461,852 outputs
Outputs from Biochemistry
#21,478
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Outputs of similar age
#285,746
of 326,507 outputs
Outputs of similar age from Biochemistry
#92
of 115 outputs
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