Title |
Flavivirus NS1 Structures Reveal Surfaces for Associations with Membranes and the Immune System
|
---|---|
Published in |
Science, February 2014
|
DOI | 10.1126/science.1247749 |
Pubmed ID | |
Authors |
David L. Akey, W. Clay Brown, Somnath Dutta, Jamie Konwerski, Joyce Jose, Thomas J. Jurkiw, James DelProposto, Craig M. Ogata, Georgios Skiniotis, Richard J. Kuhn, Janet L. Smith |
Abstract |
Flaviviruses, the human pathogens responsible for dengue fever, West Nile fever, tick-borne encephalitis, and yellow fever, are endemic in tropical and temperate parts of the world. The flavivirus nonstructural protein 1 (NS1) functions in genome replication as an intracellular dimer and in immune system evasion as a secreted hexamer. We report crystal structures for full-length, glycosylated NS1 from West Nile and dengue viruses. The NS1 hexamer in crystal structures is similar to a solution hexamer visualized by single-particle electron microscopy. Recombinant NS1 binds to lipid bilayers and remodels large liposomes into lipoprotein nanoparticles. The NS1 structures reveal distinct domains for membrane association of the dimer and interactions with the immune system and are a basis for elucidating the molecular mechanism of NS1 function. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 44% |
Australia | 2 | 22% |
Unknown | 3 | 33% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 5 | 56% |
Scientists | 3 | 33% |
Science communicators (journalists, bloggers, editors) | 1 | 11% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 3 | <1% |
Brazil | 1 | <1% |
India | 1 | <1% |
Finland | 1 | <1% |
Japan | 1 | <1% |
Mexico | 1 | <1% |
Unknown | 388 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 82 | 21% |
Researcher | 58 | 15% |
Student > Master | 51 | 13% |
Student > Bachelor | 42 | 11% |
Other | 14 | 4% |
Other | 47 | 12% |
Unknown | 102 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 82 | 21% |
Agricultural and Biological Sciences | 76 | 19% |
Immunology and Microbiology | 47 | 12% |
Chemistry | 23 | 6% |
Medicine and Dentistry | 19 | 5% |
Other | 40 | 10% |
Unknown | 109 | 28% |