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Western Blotting

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Cover of 'Western Blotting'

Table of Contents

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    Book Overview
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    Chapter 1 The Early Days of Blotting
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    Chapter 2 Origins of Protein Blotting
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    Chapter 3 Western Blotting: Remembrance of Things Past
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    Chapter 4 Simian Virus 40 and Protein Transfer
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    Chapter 5 Western Blotting: An Introduction
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    Chapter 6 From Little Helpers to Automation
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    Chapter 7 Spectrophotometric Methods to Determine Protein Concentration
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    Chapter 8 Solubilization of proteins: the importance of lysis buffer choice.
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    Chapter 9 Simultaneous Immunoblotting Analysis with Activity Gel Electrophoresis and 2-D Gel Electrophoresis
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    Chapter 10 Diffusion Blotting: A Rapid and Simple Method for Production of Multiple Blots from a Single Gel
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    Chapter 11 Multiple Immunoblots by Passive Diffusion of Proteins from a Single SDS-PAGE Gel
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    Chapter 12 Slice Blotting
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    Chapter 13 Localizing Proteins by Tissue Printing
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    Chapter 14 Dot-Immunobinding Assay (Dot-Iba)
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    Chapter 15 Analysis of Antibody Clonotype by Affinity Immunoblotting
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    Chapter 16 Glycosaminoglycan Blotting and Detection After Electrophoresis Separation
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    Chapter 17 A well-based reverse-phase protein array of formalin-fixed paraffin-embedded tissue.
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    Chapter 18 Quantitative Computerized Western Blotting in Detail
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    Chapter 19 Cationic Electrophoresis and Eastern Blotting
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    Chapter 20 A Miniaturized Blotting System for Simultaneous Detection of Different Autoantibodies
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    Chapter 21 Proteomic expressional profiling of a paraffin-embedded tissue by multiplex tissue immunoblotting.
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    Chapter 22 Post-Staining Electroblotting for Efficient and Reliable Peptide Blotting
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    Chapter 23 Multistrip Western blotting: a tool for comparative quantitative analysis of multiple proteins.
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    Chapter 24 Western Blotting Using PVDF Membranes and Its Downstream Applications
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    Chapter 25 Blotting from PhastGel to Membranes by Ultrasound
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    Chapter 26 Western Blotting of High and Low Molecular Weight Proteins Using Heat
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    Chapter 27 Membrane Strip Affinity Purification of Autoantibodies
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    Chapter 28 Strip Immunoblotting of Multiple Antigenic Peptides
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    Chapter 29 Double-Blotting: A Solution to the Problem of Nonspecific Binding of Secondary Antibodies in Immunoblotting Procedures
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    Chapter 30 Method for Resolution and Western Blotting of Very Large Proteins Using Agarose Electrophoresis
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    Chapter 31 Immunodetection of P-Selectin Using an Antibody to Its C-Terminal Tag
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    Chapter 32 Improvements and Variants of the Multiple Antigen Blot Assay-MABA: An Immunoenzymatic Technique for Simultaneous Antigen and Antibody Screening
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    Chapter 33 Blotting from Immobilized pH Gradient Gels: Application to Total Cell Lysates
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    Chapter 34 Immunoprecipitation: Western Blot for Proteins of Low Abundance
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    Chapter 35 Native Electrophoresis and Western Blot Analysis (NEWeB): Methods and Applications
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    Chapter 36 Shift-Western Blotting: Separate Analysis of Protein and DNA from Protein–DNA Complexes
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    Chapter 37 Grid-Immunoblotting
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    Chapter 38 Detection and Quantification of Protein–Protein Interactions by Far-Western Blotting
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    Chapter 39 Western Blot Analysis of Adhesive Interactions Under Fluid Shear Conditions: The Blot Rolling Assay
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    Chapter 40 Centrifuge Blotting
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    Chapter 41 Blotting of Coomassie Blue-Stained Proteins from PAGE Gels to Transparencies
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    Chapter 42 B-Cell ELISPOT: For the Identification of Antigen-Specific Antibody-Secreting Cells
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    Chapter 43 T Cell ELISPOT: For the Identification of Specific Cytokine-Secreting T Cells
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    Chapter 44 Membrane Microplates for One- and Two-Color ELISPOT and FLUOROSPOT Assays
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    Chapter 45 SDS-PAGE to Immunoblot in One Hour
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    Chapter 46 Single-cell Western blotting.
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    Chapter 47 Protein Detection by Simple Western™ Analysis
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    Chapter 48 Western Blotting Using Microfluidics
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    Chapter 49 Two-Dimensional Gel-Based Protein Standardization Verified by Western Blot Analysis
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    Chapter 50 Fingerprint Deposition on Nitrocellulose and Polyvinylidene Difluoride Membranes Using Alkaline Phosphatase
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    Chapter 51 Other Notable Protein Blotting Methods: A Brief Review
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Title
Western Blotting
Published by
Springer New York, January 2015
DOI 10.1007/978-1-4939-2694-7
ISBNs
978-1-4939-2693-0, 978-1-4939-2694-7
Editors

Biji T. Kurien, R. Hal Scofield

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The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 359 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Hong Kong 1 <1%
Unknown 358 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 57 16%
Student > Master 53 15%
Researcher 43 12%
Student > Ph. D. Student 43 12%
Other 16 4%
Other 41 11%
Unknown 106 30%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 94 26%
Agricultural and Biological Sciences 44 12%
Immunology and Microbiology 20 6%
Medicine and Dentistry 17 5%
Chemistry 16 4%
Other 56 16%
Unknown 112 31%