| Title |
Lost miRNA surveillance of Notch, IGFR pathway—road to sarcomagenesis
|
|---|---|
| Published in |
Tumor Biology, August 2013
|
| DOI | 10.1007/s13277-013-1068-5 |
| Pubmed ID | |
| Authors |
K. Galoian, T. Guettouche, B. Issac, L. Navarro, H. T. Temple |
| Abstract |
The goal of this study was to compare and analyze differentially expressed miRNA and their targets in human chondrosarcoma JJ012 and chondrocytes C 28 cell lines (control) to elucidate deregulation of major signal transduction pathways involved in sarcomagenesis. Total RNA extraction was followed by analyzing RNA quality and integrity. Exiqon human miRNA panel of 743 unique miRNA assays and Illumina microarray HT-12 platform and quantitative reverse transcriptase–PCR verification of targets were performed. The results from human miRNA Exiqon arrays with biological triplicates indicated 28 significant miRNAs (P value ≤0.01). A total 3,045 target genes were derived from the miRWalk database for these 28 miRNAs with 587 common and 2,458 unique target genes. The results of our analyses of the significantly downregulated and upregulated miRNAs in chondrosarcoma cell line indicated the predominant dysregulation of NOTCH, insulin-like growth factor receptor (IGFR), and downstream rat sarcoma, and Src pathways, compared to control. Among the upregulated targets for upregulated miRNAs were the cluster of cancer testis antigen (CTA) genes, located on X chromosome, and their expression was correlated to IGFR pathway activity. Based on our observations, lost miRNA surveillance of NOTCH and IGFR pathways is involved in and leads to sarcomagenesis. We conclude that upregulation of CTA genes is due to hypomethylation that are controlled by epi-miRNAs. We do not preclude the possibility that the upregulated miRNAs, which target CTA genes located in adjacent regions in chromosome X, are epi-miRNAs that influence target gene expression by directly regulating epigenetic processes. Future endeavors will be directed towards understanding the posttranscriptional modifications that affect miRNA expression in sarcomas. |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 5% |
| Unknown | 19 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 30% |
| Researcher | 5 | 25% |
| Student > Ph. D. Student | 4 | 20% |
| Student > Bachelor | 1 | 5% |
| Other | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 2 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 50% |
| Medicine and Dentistry | 3 | 15% |
| Biochemistry, Genetics and Molecular Biology | 2 | 10% |
| Computer Science | 1 | 5% |
| Environmental Science | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 1 | 5% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 August 2022.
All research outputs
#2,980,744
of 23,053,169 outputs
Outputs from Tumor Biology
#57
of 2,630 outputs
Outputs of similar age
#26,815
of 199,435 outputs
Outputs of similar age from Tumor Biology
#3
of 27 outputs
Altmetric has tracked 23,053,169 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,630 research outputs from this source. They receive a mean Attention Score of 2.3. This one has done particularly well, scoring higher than 97% of its peers.
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We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.