| Title |
Using optogenetics to assess neuroendocrine modulation of heart rate in Drosophila melanogaster larvae
|
|---|---|
| Published in |
Journal of Comparative Physiology A, June 2017
|
| DOI | 10.1007/s00359-017-1191-7 |
| Pubmed ID | |
| Authors |
Cole Malloy, Jacob Sifers, Angela Mikos, Aya Samadi, Aya Omar, Christina Hermanns, Robin L. Cooper |
| Abstract |
The Drosophila melanogaster heart has become a principal model in which to study cardiac physiology and development. While the morphology of the heart in Drosophila and mammals is different, many of the molecular mechanisms that underlie heart development and function are similar and function can be assessed by similar physiological measurements, such as cardiac output, rate, and time in systole or diastole. Here, we have utilized an intact, optogenetic approach to assess the neural influence on heart rate in the third instar larvae. To simulate the release of modulators from the nervous system in response to environmental influences, we have directed expression of channel-rhodopsin variants to targeted neuronal populations to assess the role of these neural ensembles in directing release of modulators that may affect heart rate in vivo. Our observations show that the activation of targeted neurons, including cholinergic, dopaminergic, and serotonergic neurons, stimulate the release of cardioactive substances that increase heart rate after the initial activation at both room temperature and in a cold environment. This parallels previous studies suggesting these modulators play a crucial role in altering heart rate when applied to exposed hearts and adds to our understanding of chemical modulation of heart rate in intact Drosophila larvae. |
X Demographics
The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 57% |
| United Kingdom | 2 | 29% |
| Unknown | 1 | 14% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 57% |
| Scientists | 3 | 43% |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 27 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 19% |
| Student > Ph. D. Student | 4 | 15% |
| Student > Bachelor | 3 | 11% |
| Student > Postgraduate | 2 | 7% |
| Professor > Associate Professor | 2 | 7% |
| Other | 3 | 11% |
| Unknown | 8 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 22% |
| Neuroscience | 6 | 22% |
| Engineering | 3 | 11% |
| Agricultural and Biological Sciences | 1 | 4% |
| Medicine and Dentistry | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 9 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 July 2018.
All research outputs
#7,856,604
of 23,815,455 outputs
Outputs from Journal of Comparative Physiology A
#468
of 1,450 outputs
Outputs of similar age
#122,091
of 318,900 outputs
Outputs of similar age from Journal of Comparative Physiology A
#10
of 20 outputs
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So far Altmetric has tracked 1,450 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.9. This one is in the 37th percentile – i.e., 37% of its peers scored the same or lower than it.
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We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.