| Title |
Prion-Like Seeding of Misfolded α-Synuclein in the Brains of Dementia with Lewy Body Patients in RT-QUIC
|
|---|---|
| Published in |
Molecular Neurobiology, May 2017
|
| DOI | 10.1007/s12035-017-0624-1 |
| Pubmed ID | |
| Authors |
Kazunori Sano, Ryuichiro Atarashi, Katsuya Satoh, Daisuke Ishibashi, Takehiro Nakagaki, Yasushi Iwasaki, Mari Yoshida, Shigeo Murayama, Kenichi Mishima, Noriyuki Nishida |
| Abstract |
The prion-like seeding of misfolded α-synuclein (αSyn) involved in the pathogenesis of Lewy body diseases (LBD) remains poorly understood at the molecular level. Using the real-time quaking-induced conversion (RT-QUIC) seeding assay, we investigated whether brain tissues from cases of dementia with Lewy bodies (DLB), which contain serine 129 (Ser129)-phosphorylated insoluble aggregates of αSyn, can convert Escherichia coli-derived recombinant αSyn (r-αSyn) to fibrils. Diffuse neocortical DLB yielded 50% seeding dose (SD50) values of 10(7)~10(10)/g brain. Limbic DLB was estimated to have an SD50 value of ~10(5)/g brain. Furthermore, RT-QUIC assay discriminated DLB from other neurological and neurodegenerative disorders. Unexpectedly, the prion-like seeding was reconstructed in reactions seeded with oligomer-like species, but not with insoluble aggregates of r-αSyn, regardless of Ser129 phosphorylation status. Our findings suggest that RT-QUIC using r-αSyn can be applied to detect seeding activity in LBD, and the culprit that causes prion-like seeding may be oligomeric forms of αSyn. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 33% |
| Canada | 1 | 33% |
| United States | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 108 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | <1% |
| Unknown | 107 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 25 | 23% |
| Student > Ph. D. Student | 21 | 19% |
| Student > Master | 9 | 8% |
| Student > Doctoral Student | 7 | 6% |
| Student > Bachelor | 7 | 6% |
| Other | 16 | 15% |
| Unknown | 23 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 27 | 25% |
| Biochemistry, Genetics and Molecular Biology | 21 | 19% |
| Agricultural and Biological Sciences | 11 | 10% |
| Medicine and Dentistry | 10 | 9% |
| Psychology | 4 | 4% |
| Other | 9 | 8% |
| Unknown | 26 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 July 2021.
All research outputs
#2,845,274
of 22,981,247 outputs
Outputs from Molecular Neurobiology
#417
of 3,481 outputs
Outputs of similar age
#54,203
of 313,454 outputs
Outputs of similar age from Molecular Neurobiology
#9
of 124 outputs
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So far Altmetric has tracked 3,481 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one has done well, scoring higher than 87% of its peers.
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