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Directed Evolution Library Creation

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Cover of 'Directed Evolution Library Creation'

Table of Contents

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    Book Overview
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    Chapter 1 Directed Evolution Library Creation
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    Chapter 2 Error-Prone Rolling Circle Amplification Greatly Simplifies Random Mutagenesis
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    Chapter 3 Random Mutagenesis by Error-Prone Pol Plasmid Replication in Escherichia coli.
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    Chapter 4 The Sequence Saturation Mutagenesis (SeSaM) Method
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    Chapter 5 Generation of Effective Libraries by Neutral Drift
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    Chapter 6 Site-Saturation Mutagenesis by Overlap Extension PCR
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    Chapter 7 Iterative saturation mutagenesis: a powerful approach to engineer proteins by systematically simulating darwinian evolution.
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    Chapter 8 Generating Targeted Libraries by the Combinatorial Incorporation of Synthetic Oligonucleotides During Gene Shuffling (ISOR)
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    Chapter 9 OmniChange: Simultaneous Site Saturation of Up to Five Codons
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    Chapter 10 Random Insertional–Deletional Strand Exchange Mutagenesis (RAISE): A Simple Method for Generating Random Insertion and Deletion Mutations
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    Chapter 11 Transposon-based approaches for generating novel molecular diversity during directed evolution.
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    Chapter 12 Restriction Enzyme-Mediated DNA Family Shuffling
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    Chapter 13 Assembly of Designed Oligonucleotides: A Useful Tool in Synthetic Biology for Creating High-Quality Combinatorial DNA Libraries
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    Chapter 14 One-Pot Simple Methodology for Cassette Randomization and Recombination for Focused Directed Evolution (OSCARR)
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    Chapter 15 USER Friendly DNA Recombination (USERec): Gene Library Construction Requiring Minimal Sequence Homology.
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    Chapter 16 ITCHY: Incremental Truncation for the Creation of Hybrid Enzymes
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    Chapter 17 Generating Random Circular Permutation Libraries
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    Chapter 18 Probabilistic Methods in Directed Evolution: Library Size, Mutation Rate, and Diversity
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    Chapter 19 The Mutagenesis Assistant Program
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    Chapter 20 Computational Tools for Designing Smart Libraries
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    Chapter 21 Computational tools for directed evolution: a comparison of prospective and retrospective strategies.
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    Chapter 22 Designing Libraries of Chimeric Proteins Using SCHEMA Recombination and RASPP
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    Chapter 23 Noncontiguous SCHEMA Protein Recombination.
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    Chapter 24 Engineering Proteins by Reconstructing Evolutionary Adaptive Paths
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Title
Directed Evolution Library Creation
Published by
Springer New York, July 2014
DOI 10.1007/978-1-4939-1053-3
ISBNs
978-1-4939-1052-6, 978-1-4939-1053-3
Editors

Gillam, Elizabeth M.J., Copp, Janine N., Ackerley, David

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 116 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 <1%
United States 1 <1%
France 1 <1%
Canada 1 <1%
Unknown 112 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 28 24%
Student > Bachelor 18 16%
Researcher 16 14%
Student > Master 16 14%
Student > Doctoral Student 11 9%
Other 15 13%
Unknown 12 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 39 34%
Biochemistry, Genetics and Molecular Biology 38 33%
Chemistry 10 9%
Engineering 5 4%
Medicine and Dentistry 3 3%
Other 6 5%
Unknown 15 13%