Title |
The spliceosome: disorder and dynamics defined
|
---|---|
Published in |
Current Opinion in Structural Biology, February 2014
|
DOI | 10.1016/j.sbi.2014.01.009 |
Pubmed ID | |
Authors |
Weijun Chen, Melissa J Moore |
Abstract |
Among the many macromolecular machines involved in eukaryotic gene expression, the spliceosome remains one of the most challenging for structural biologists. Defining features of this highly complex apparatus are its excessive number of individual parts, many of which have been evolutionarily selected for regions of structural disorder, and the remarkable compositional and conformation dynamics it must undertake to complete each round of splicing. Here we review recent advances in our understanding of spliceosome structural dynamics stemming from bioinformatics, deep sequencing, high throughput methods for determining protein-protein, protein-RNA and RNA-RNA interaction dynamics, single molecule microscopy and more traditional structural analyses. Together, these tools are rapidly changing our structural appreciation of this remarkably dynamic machine. |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
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Sweden | 1 | <1% |
Unknown | 129 | 99% |
Demographic breakdown
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Student > Ph. D. Student | 39 | 30% |
Researcher | 33 | 25% |
Student > Master | 20 | 15% |
Professor > Associate Professor | 6 | 5% |
Student > Doctoral Student | 4 | 3% |
Other | 7 | 5% |
Unknown | 21 | 16% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 3 | 2% |
Medicine and Dentistry | 3 | 2% |
Other | 6 | 5% |
Unknown | 27 | 21% |