| Title |
Epigenetic Modulation of Adult Hippocampal Neurogenesis by Extremely Low-Frequency Electromagnetic Fields
|
|---|---|
| Published in |
Molecular Neurobiology, February 2014
|
| DOI | 10.1007/s12035-014-8650-8 |
| Pubmed ID | |
| Authors |
Lucia Leone, Salvatore Fusco, Alessia Mastrodonato, Roberto Piacentini, Saviana Antonella Barbati, Salvatore Zaffina, Giovambattista Pani, Maria Vittoria Podda, Claudio Grassi |
| Abstract |
Throughout life, adult neurogenesis generates new neurons in the dentate gyrus of hippocampus that have a critical role in memory formation. Strategies able to stimulate this endogenous process have raised considerable interest because of their potential use to treat neurological disorders entailing cognitive impairment. We previously reported that mice exposed to extremely low-frequency electromagnetic fields (ELFEFs) showed increased hippocampal neurogenesis. Here, we demonstrate that the ELFEF-dependent enhancement of hippocampal neurogenesis improves spatial learning and memory. To gain insights on the molecular mechanisms underlying ELFEFs' effects, we extended our studies to an in vitro model of neural stem cells (NSCs) isolated from the hippocampi of newborn mice. We found that ELFEFs enhanced proliferation and neuronal differentiation of hippocampal NSCs by regulation of epigenetic mechanisms leading to pro-neuronal gene expression. Upon ELFEF stimulation of NSCs, we observed a significant enhancement of expression of the pro-proliferative gene hairy enhancer of split 1 and the neuronal determination genes NeuroD1 and Neurogenin1. These events were preceded by increased acetylation of H3K9 and binding of the phosphorylated transcription factor cAMP response element-binding protein (CREB) on the regulatory sequence of these genes. Such ELFEF-dependent epigenetic modifications were prevented by the Cav1-channel blocker nifedipine, and were associated with increased occupancy of CREB-binding protein (CBP) to the same loci within the analyzed promoters. Our results unravel the molecular mechanisms underlying the ELFEFs' ability to improve endogenous neurogenesis, pointing to histone acetylation-related chromatin remodeling as a critical determinant. These findings could pave the way to the development of novel therapeutic approaches in regenerative medicine. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 20% |
| Canada | 1 | 20% |
| Unknown | 3 | 60% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 80% |
| Scientists | 1 | 20% |
Mendeley readers
The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Italy | 1 | 1% |
| Ireland | 1 | 1% |
| Switzerland | 1 | 1% |
| Unknown | 66 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 19% |
| Researcher | 12 | 17% |
| Student > Bachelor | 7 | 10% |
| Student > Master | 6 | 9% |
| Professor > Associate Professor | 5 | 7% |
| Other | 14 | 20% |
| Unknown | 12 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 13 | 19% |
| Biochemistry, Genetics and Molecular Biology | 8 | 12% |
| Medicine and Dentistry | 8 | 12% |
| Agricultural and Biological Sciences | 7 | 10% |
| Psychology | 4 | 6% |
| Other | 10 | 14% |
| Unknown | 19 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 April 2019.
All research outputs
#4,162,594
of 22,745,803 outputs
Outputs from Molecular Neurobiology
#863
of 3,434 outputs
Outputs of similar age
#41,932
of 222,819 outputs
Outputs of similar age from Molecular Neurobiology
#5
of 27 outputs
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So far Altmetric has tracked 3,434 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one has gotten more attention than average, scoring higher than 69% of its peers.
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