| Title |
Primary culture of avian embryonic heart forming region cells to study the regulation of vertebrate early heart morphogenesis by vitamin A
|
|---|---|
| Published in |
BMC Developmental Biology, February 2014
|
| DOI | 10.1186/1471-213x-14-10 |
| Pubmed ID | |
| Authors |
Inese Cakstina, Una Riekstina, Martins Boroduskis, Ilva Nakurte, Janis Ancans, Maija H Zile, Indrikis Muiznieks |
| Abstract |
Important knowledge about the role of vitamin A in vertebrate heart development has been obtained using the vitamin A-deficient avian in ovo model which enables the in vivo examination of very early stages of vertebrate heart morphogenesis. These studies have revealed the critical role of the vitamin A-active form, retinoic acid (RA) in the regulation of several developmental genes, including the important growth regulatory factor, transforming growth factor-beta2 (TGFβ2), involved in early events of heart morphogenesis. However, this in ovo model is not readily available for elucidating details of molecular mechanisms determining RA activity, thus limiting further examination of RA-regulated early heart morphogenesis. In order to obtain insights into RA-regulated gene expression during these early events, a reliable in vitro model is needed. Here we describe a cell culture that closely reproduces the in ovo observed regulatory effects of RA on TGFβ2 and on several developmental genes linked to TGFβ signaling during heart morphogenesis. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 33% |
| Scientists | 1 | 33% |
| Members of the public | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| France | 1 | 2% |
| Unknown | 39 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 15% |
| Student > Bachelor | 5 | 12% |
| Student > Ph. D. Student | 5 | 12% |
| Professor > Associate Professor | 4 | 10% |
| Student > Master | 3 | 7% |
| Other | 4 | 10% |
| Unknown | 14 | 34% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 29% |
| Biochemistry, Genetics and Molecular Biology | 8 | 20% |
| Medicine and Dentistry | 4 | 10% |
| Engineering | 2 | 5% |
| Chemistry | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 13 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 February 2014.
All research outputs
#15,281,478
of 23,486,774 outputs
Outputs from BMC Developmental Biology
#247
of 372 outputs
Outputs of similar age
#130,527
of 225,642 outputs
Outputs of similar age from BMC Developmental Biology
#5
of 6 outputs
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