Title |
MicroRNA-34a dependent regulation of AXL controls the activation of dendritic cells in inflammatory arthritis
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Published in |
Nature Communications, June 2017
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DOI | 10.1038/ncomms15877 |
Pubmed ID | |
Authors |
Mariola Kurowska-Stolarska, Stefano Alivernini, Emma Garcia Melchor, Aziza Elmesmari, Barbara Tolusso, Clare Tange, Luca Petricca, Derek S. Gilchrist, Gabriele Di Sante, Chantal Keijzer, Lynn Stewart, Clara Di Mario, Vicky Morrison, James M. Brewer, Duncan Porter, Simon Milling, Ronald D. Baxter, David McCarey, Elisa Gremese, Greg Lemke, Gianfranco Ferraccioli, Charles McSharry, Iain B. McInnes |
Abstract |
Current treatments for rheumatoid arthritis (RA) do not reverse underlying aberrant immune function. A genetic predisposition to RA, such as HLA-DR4 positivity, indicates that dendritic cells (DC) are of crucial importance to pathogenesis by activating auto-reactive lymphocytes. Here we show that microRNA-34a provides homoeostatic control of CD1c(+) DC activation via regulation of tyrosine kinase receptor AXL, an important inhibitory DC auto-regulator. This pathway is aberrant in CD1c(+) DCs from patients with RA, with upregulation of miR-34a and lower levels of AXL compared to DC from healthy donors. Production of pro-inflammatory cytokines is reduced by ex vivo gene-silencing of miR-34a. miR-34a-deficient mice are resistant to collagen-induced arthritis and interaction of DCs and T cells from these mice are reduced and do not support the development of Th17 cells in vivo. Our findings therefore show that miR-34a is an epigenetic regulator of DC function that may contribute to RA. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
India | 1 | 14% |
Italy | 1 | 14% |
Unknown | 5 | 71% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 5 | 71% |
Science communicators (journalists, bloggers, editors) | 1 | 14% |
Practitioners (doctors, other healthcare professionals) | 1 | 14% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 78 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 15 | 19% |
Researcher | 13 | 17% |
Student > Bachelor | 9 | 12% |
Student > Doctoral Student | 6 | 8% |
Professor | 6 | 8% |
Other | 15 | 19% |
Unknown | 14 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 15 | 19% |
Biochemistry, Genetics and Molecular Biology | 13 | 17% |
Immunology and Microbiology | 12 | 15% |
Agricultural and Biological Sciences | 10 | 13% |
Pharmacology, Toxicology and Pharmaceutical Science | 5 | 6% |
Other | 8 | 10% |
Unknown | 15 | 19% |