| Title |
Carbon Nanotube Reinforced Collagen/Hydroxyapatite Scaffolds Improve Bone Tissue Formation In Vitro and In Vivo
|
|---|---|
| Published in |
Annals of Biomedical Engineering, June 2017
|
| DOI | 10.1007/s10439-017-1866-9 |
| Pubmed ID | |
| Authors |
Zheng Jing, Yeke Wu, Wen Su, Mi Tian, Wenlu Jiang, Li Cao, Lixing Zhao, Zhihe Zhao |
| Abstract |
Current bone regeneration strategies faced major challenges in fabricating the bionic scaffolds with nano-structure, constituents and mechanical features of native bone. In this study, we developed a new porous scaffold by adding the multi-walled carbon nanotube (MWCNT) into collagen (Col)/hydroxyapatite (HA) composites. Data showed that 0.5%CNT/Col/HA (0.5%CNT) group was approximately tenfolds stiffer than Col-HA, and it was superior in promoting bone marrow mesenchymal stem proliferation and spreading, mRNA and protein expressions of bone sialoprotein (BSP) and osteocalcin (OCN) than Col-HA group. Moreover, we utilized 0.5%CNT composite to repair the rat calvarial defects (8 mm diameter) in vivo, and observed the new bone formation by 3D reconstruction of micro CT, HE and Masson staining, and BSP, OCN by immunohistochemical analysis. Results showed that newly formed bone in 0.5%CNT group was significantly higher than that in Col-HA group at 12 weeks. These findings highlighted a promising strategy in healing of large area bone defect with MWCNT added into the Col-HA scaffold as they possessed the combined effects of mechanical strength and osteogenicity. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 60% |
| Science communicators (journalists, bloggers, editors) | 2 | 40% |
Mendeley readers
The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 67 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 10 | 15% |
| Researcher | 8 | 12% |
| Student > Doctoral Student | 7 | 10% |
| Student > Ph. D. Student | 6 | 9% |
| Student > Bachelor | 5 | 7% |
| Other | 5 | 7% |
| Unknown | 26 | 39% |
| Readers by discipline | Count | As % |
|---|---|---|
| Materials Science | 11 | 16% |
| Engineering | 9 | 13% |
| Medicine and Dentistry | 5 | 7% |
| Chemical Engineering | 4 | 6% |
| Chemistry | 3 | 4% |
| Other | 9 | 13% |
| Unknown | 26 | 39% |