| Title |
An improved method suitable for isolation of high-quality metagenomic DNA from diverse soils
|
|---|---|
| Published in |
3 Biotech, June 2017
|
| DOI | 10.1007/s13205-017-0847-x |
| Pubmed ID | |
| Authors |
Sumit Kumar Verma, Himanshi Singh, Prakash C. Sharma |
| Abstract |
Standardization of metagenomic DNA extraction protocol is a pre-requisite for a successful metagenomic study aiming to screen and exploit the variety of microorganisms inhabiting a particular soil environment. Six methods reported earlier were used for isolation of metagenomic DNA in the present study. These methods suffered with regard to either poor yield or quality of DNA. Therefore, we developed an improved method for isolation of high-molecular weight and good quality metagenomic DNA from different soil samples. Our protocol combines the enzymatic (lysozyme and proteinase K) and chemical (CTAB and CaCl2) strategies to ensure efficient cell lysis and use of PEG and isopropanol for precipitation of humic impurities-free DNA. Our improved method gave high yield of good quality metagenomic DNA from diverse soils collected from garden, domestic waste dumping site, cellulose waste dumping site, sewage site, and tannery waste site. The good quality of the metagenomic DNA was evident by spectrophotometry data, PCR amplification of 16S rRNA gene and restriction digestion. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 20% |
| Poland | 1 | 20% |
| Unknown | 3 | 60% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 80% |
| Scientists | 1 | 20% |
Mendeley readers
The data shown below were compiled from readership statistics for 138 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 138 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 24 | 17% |
| Researcher | 19 | 14% |
| Student > Bachelor | 14 | 10% |
| Student > Ph. D. Student | 12 | 9% |
| Student > Doctoral Student | 10 | 7% |
| Other | 15 | 11% |
| Unknown | 44 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 37 | 27% |
| Agricultural and Biological Sciences | 33 | 24% |
| Environmental Science | 6 | 4% |
| Engineering | 3 | 2% |
| Immunology and Microbiology | 3 | 2% |
| Other | 9 | 7% |
| Unknown | 47 | 34% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 March 2023.
All research outputs
#14,691,575
of 25,516,314 outputs
Outputs from 3 Biotech
#298
of 1,437 outputs
Outputs of similar age
#162,026
of 328,605 outputs
Outputs of similar age from 3 Biotech
#24
of 87 outputs
Altmetric has tracked 25,516,314 research outputs across all sources so far. This one is in the 41st percentile – i.e., 41% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,437 research outputs from this source. They receive a mean Attention Score of 3.1. This one has done well, scoring higher than 78% of its peers.
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We're also able to compare this research output to 87 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.