Title |
Bone metabolism markers and angiogenic cytokines as regulators of human hematopoietic stem cell mobilization
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Published in |
Journal of Bone and Mineral Metabolism, June 2017
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DOI | 10.1007/s00774-017-0853-4 |
Pubmed ID | |
Authors |
Pantelis Tsirkinidis, Evangelos Terpos, Georgios Boutsikas, Athanasios Papatheodorou, Konstantinos Anargyrou, Eleni Lalou, Aglaia Dimitrakopoulou, Christina Kalpadakis, Konstantinos Konstantopoulos, Marina Siakantaris, Panayiotis Panayiotidis, Gerassimos Pangalis, Marie-Christine Kyrtsonis, Theodoros Vassilakopoulos, Maria K. Angelopoulou |
Abstract |
Hematopoietic stem cell (HSC) mobilization involves cleavage of ligands between HSC and niche components. However, there are scarce data regarding the role of bone cells in human HSC mobilization. We studied biochemical markers of bone metabolism and angiogenic cytokines during HSC mobilization in 46 patients' sera with lymphoma and multiple myeloma, by ELISA. Significant changes between pre-mobilization and collection samples were found: (1) Bone alkaline phosphatase (BALP) increased, indicating augmentation of bone formation; (2) Receptor activator of Nf-κB ligand/osteoprotegerin ratio (RANKL/OPG) increased, showing osteoclastic differentiation and survival; however, there was no evidence of increased osteoclastic activity; and (3) Angiopoietin-1/Angiopoietin-2 ratio (ANGP-1/ANGP-2) decreased, consistent with vessel destabilization. Poor mobilizers had significantly higher carboxy-terminal telopeptide of collagen type I (CTX) and lower ANGP-1 at pre-mobilization samples, compared to good ones. CTX, amino-terminal telopeptide of collagen type I (NTX) and ANGP-1 pre-mobilization levels correlated significantly with circulating CD34(+) peak cell counts. Our results indicate that bone formation and vessel destabilization are the two major events during human HSC mobilization. Osteoblasts seem to be the orchestrating cells, while osteoclasts are stimulated but not fully active. Moreover, ANGP-1, CTX and NTX may serve as predictors of poor mobilization. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 19 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Other | 4 | 21% |
Student > Ph. D. Student | 3 | 16% |
Student > Master | 3 | 16% |
Professor | 1 | 5% |
Lecturer | 1 | 5% |
Other | 1 | 5% |
Unknown | 6 | 32% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 6 | 32% |
Agricultural and Biological Sciences | 2 | 11% |
Sports and Recreations | 2 | 11% |
Immunology and Microbiology | 1 | 5% |
Engineering | 1 | 5% |
Other | 0 | 0% |
Unknown | 7 | 37% |