↓ Skip to main content
What is this page? Embed badge

Bacterial Protein Secretion Systems

Overview of attention for book
Cover of 'Bacterial Protein Secretion Systems'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Identification of Protein Secretion Systems in Bacterial Genomes Using MacSyFinder
  3. Altmetric Badge
    Chapter 2 Protein Sorting Prediction
  4. Altmetric Badge
    Chapter 3 Cell Fractionation
  5. Altmetric Badge
    Chapter 4 Defining Lipoprotein Localisation by Fluorescence Microscopy
  6. Altmetric Badge
    Chapter 5 Identification of Lipoproteins Using Globomycin and Radioactive Palmitate
  7. Altmetric Badge
    Chapter 6 Defining Membrane Protein Localization by Isopycnic Density Gradients
  8. Altmetric Badge
    Chapter 7 Cell Surface Exposure
  9. Altmetric Badge
    Chapter 8 Probing Inner Membrane Protein Topology by Proteolysis
  10. Altmetric Badge
    Chapter 9 Mapping of Membrane Protein Topology by Substituted Cysteine Accessibility Method (SCAM™)
  11. Altmetric Badge
    Chapter 10 Defining Membrane Protein Topology Using pho-lac Reporter Fusions
  12. Altmetric Badge
    Chapter 11 In Vivo and In Vitro Protein–Peptidoglycan Interactions
  13. Altmetric Badge
    Chapter 12 Measure of Peptidoglycan Hydrolase Activity
  14. Altmetric Badge
    Chapter 13 Protein–Protein Interaction: Bacterial Two-Hybrid
  15. Altmetric Badge
    Chapter 14 Protein–Protein Interactions: Yeast Two-Hybrid System
  16. Altmetric Badge
    Chapter 15 Protein–Protein Interactions: Cytology Two-Hybrid
  17. Altmetric Badge
    Chapter 16 Fusion Reporter Approaches to Monitoring Transmembrane Helix Interactions in Bacterial Membranes
  18. Altmetric Badge
    Chapter 17 Protein–Protein Interactions: Co-Immunoprecipitation
  19. Altmetric Badge
    Chapter 18 Protein–Protein Interaction: Tandem Affinity Purification in Bacteria
  20. Altmetric Badge
    Chapter 19 Site-Directed and Time-Resolved Photocrosslinking in Cells Metabolically Labeled with Radioisotopes
  21. Altmetric Badge
    Chapter 20 Protein–Protein Interactions: Pull-Down Assays
  22. Altmetric Badge
    Chapter 21 Protein–Protein Interactions: Surface Plasmon Resonance
  23. Altmetric Badge
    Chapter 22 Assessing Energy-Dependent Protein Conformational Changes in the TonB System
  24. Altmetric Badge
    Chapter 23 Defining Assembly Pathways by Fluorescence Microscopy
  25. Altmetric Badge
    Chapter 24 Large Complexes: Cloning Strategy, Production, and Purification
  26. Altmetric Badge
    Chapter 25 Shearing and Enrichment of Extracellular Type IV Pili
  27. Altmetric Badge
    Chapter 26 Blue Native PAGE Analysis of Bacterial Secretion Complexes
  28. Altmetric Badge
    Chapter 27 In Situ Imaging of Bacterial Secretion Systems by Electron Cryotomography
  29. Altmetric Badge
    Chapter 28 Structural Analysis of Protein Complexes by Cryo Electron Microscopy
  30. Altmetric Badge
    Chapter 29 Bacterial Filamentous Appendages Investigated by Solid-State NMR Spectroscopy
  31. Altmetric Badge
    Chapter 30 Energy Requirements for Protein Secretion via the Flagellar Type III Secretion System
  32. Altmetric Badge
    Chapter 31 Identification of Effectors: Precipitation of Supernatant Material
  33. Altmetric Badge
    Chapter 32 Screening for Secretion of the Type VI Secretion System Protein Hcp by Enzyme-Linked Immunosorbent Assay and Colony Blot
  34. Altmetric Badge
    Chapter 33 Effector Translocation: Cya Reporter Assay
  35. Altmetric Badge
    Chapter 34 Monitoring Effector Translocation using the TEM-1 Beta-Lactamase Reporter System
  36. Altmetric Badge
    Chapter 35 Effector Translocation Assay: Differential Solubilization
  37. Altmetric Badge
    Chapter 36 Quantitative Determination of Anti-bacterial Activity During Bacterial Co-culture
  38. Altmetric Badge
    Chapter 37 Erratum to: Bacterial Filamentous Appendages Investigated by Solid-State NMR Spectroscopy
Attention for Chapter 33: Effector Translocation: Cya Reporter Assay
Altmetric Badge

About this Attention Score

  • Good Attention Score compared to outputs of the same age (65th percentile)
  • High Attention Score compared to outputs of the same age and source (90th percentile)

Mentioned by

twitter
4 X users

Citations

dimensions_citation
10 Dimensions

Readers on

mendeley
9 Mendeley
Summary X Dimensions citations
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Effector Translocation: Cya Reporter Assay
Chapter number 33
Book title
Bacterial Protein Secretion Systems
Published in
Methods in molecular biology, July 2017
DOI 10.1007/978-1-4939-7033-9_33
Pubmed ID
28667631
Book ISBNs
978-1-4939-7031-5, 978-1-4939-7033-9
Authors

Chakravarthy, Suma, Huot, Bethany, Kvitko, Brian H., Suma Chakravarthy, Bethany Huot, Brian H. Kvitko

Editors

Laure Journet, Eric Cascales

Abstract

An accurate and complete roster of the Type III effector (T3E) proteins translocated by the P. syringae Type III secretion system (T3SS) into host cells is critical to understanding the pathogen's interactions with plants. The adenylate cyclase (Cya) reporter offers a highly sensitive and robust assay for monitoring the translocation of T3Es. T3Es are fused to the calmodulin-dependent adenylate-cyclase domain of CyaA. The T3E targets Cya for translocation through the T3SS into the host cell at which point it is activated by calmodulin and converts adenosine triphosphate into cyclic adenosine monophosphate (cAMP). The T3SS translocation-dependent increase in cAMP concentration in plant cells is then measured with an enzyme-linked immunosorbent assay kit. The Cya reporter can be used to determine whether a candidate protein is translocated by T3SS or to measure relative levels of T3SS translocation in a semiquantitative manner.

View on publisher site
X Demographics

X Demographics

The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 33%
Researcher 2 22%
Student > Doctoral Student 1 11%
Student > Master 1 11%
Unknown 2 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 33%
Agricultural and Biological Sciences 2 22%
Computer Science 1 11%
Immunology and Microbiology 1 11%
Unknown 2 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 April 2018.
All research outputs
#6,913,516
of 23,312,088 outputs
Outputs from Methods in molecular biology
#2,070
of 13,318 outputs
Outputs of similar age
#107,318
of 314,905 outputs
Outputs of similar age from Methods in molecular biology
#26
of 270 outputs
Altmetric has tracked 23,312,088 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 13,318 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 314,905 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 270 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.

This page is provided by Altmetric.