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Bacterial Protein Secretion Systems

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Cover of 'Bacterial Protein Secretion Systems'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Identification of Protein Secretion Systems in Bacterial Genomes Using MacSyFinder
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    Chapter 2 Protein Sorting Prediction
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    Chapter 3 Cell Fractionation
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    Chapter 4 Defining Lipoprotein Localisation by Fluorescence Microscopy
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    Chapter 5 Identification of Lipoproteins Using Globomycin and Radioactive Palmitate
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    Chapter 6 Defining Membrane Protein Localization by Isopycnic Density Gradients
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    Chapter 7 Cell Surface Exposure
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    Chapter 8 Probing Inner Membrane Protein Topology by Proteolysis
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    Chapter 9 Mapping of Membrane Protein Topology by Substituted Cysteine Accessibility Method (SCAM™)
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    Chapter 10 Defining Membrane Protein Topology Using pho-lac Reporter Fusions
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    Chapter 11 In Vivo and In Vitro Protein–Peptidoglycan Interactions
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    Chapter 12 Measure of Peptidoglycan Hydrolase Activity
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    Chapter 13 Protein–Protein Interaction: Bacterial Two-Hybrid
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    Chapter 14 Protein–Protein Interactions: Yeast Two-Hybrid System
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    Chapter 15 Protein–Protein Interactions: Cytology Two-Hybrid
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    Chapter 16 Fusion Reporter Approaches to Monitoring Transmembrane Helix Interactions in Bacterial Membranes
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    Chapter 17 Protein–Protein Interactions: Co-Immunoprecipitation
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    Chapter 18 Protein–Protein Interaction: Tandem Affinity Purification in Bacteria
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    Chapter 19 Site-Directed and Time-Resolved Photocrosslinking in Cells Metabolically Labeled with Radioisotopes
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    Chapter 20 Protein–Protein Interactions: Pull-Down Assays
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    Chapter 21 Protein–Protein Interactions: Surface Plasmon Resonance
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    Chapter 22 Assessing Energy-Dependent Protein Conformational Changes in the TonB System
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    Chapter 23 Defining Assembly Pathways by Fluorescence Microscopy
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    Chapter 24 Large Complexes: Cloning Strategy, Production, and Purification
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    Chapter 25 Shearing and Enrichment of Extracellular Type IV Pili
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    Chapter 26 Blue Native PAGE Analysis of Bacterial Secretion Complexes
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    Chapter 27 In Situ Imaging of Bacterial Secretion Systems by Electron Cryotomography
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    Chapter 28 Structural Analysis of Protein Complexes by Cryo Electron Microscopy
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    Chapter 29 Bacterial Filamentous Appendages Investigated by Solid-State NMR Spectroscopy
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    Chapter 30 Energy Requirements for Protein Secretion via the Flagellar Type III Secretion System
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    Chapter 31 Identification of Effectors: Precipitation of Supernatant Material
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    Chapter 32 Screening for Secretion of the Type VI Secretion System Protein Hcp by Enzyme-Linked Immunosorbent Assay and Colony Blot
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    Chapter 33 Effector Translocation: Cya Reporter Assay
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    Chapter 34 Monitoring Effector Translocation using the TEM-1 Beta-Lactamase Reporter System
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    Chapter 35 Effector Translocation Assay: Differential Solubilization
  37. Altmetric Badge
    Chapter 36 Quantitative Determination of Anti-bacterial Activity During Bacterial Co-culture
  38. Altmetric Badge
    Chapter 37 Erratum to: Bacterial Filamentous Appendages Investigated by Solid-State NMR Spectroscopy
Attention for Chapter 24: Large Complexes: Cloning Strategy, Production, and Purification
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Chapter title
Large Complexes: Cloning Strategy, Production, and Purification
Chapter number 24
Book title
Bacterial Protein Secretion Systems
Published in
Methods in molecular biology, July 2017
DOI 10.1007/978-1-4939-7033-9_24
Pubmed ID
Book ISBNs
978-1-4939-7031-5, 978-1-4939-7033-9
Authors

Durand, Eric, Lloubes, Roland, Eric Durand, Roland Lloubes, Roland Lloubès

Editors

Laure Journet, Eric Cascales

Abstract

Membrane proteins can assemble and form complexes in the cell envelope. In Gram-negative bacteria, a number of multiprotein complexes, including secretion systems, efflux pumps, molecular motors, and pilus assembly machines, comprise proteins from the inner and outer membranes. Besides the structures of isolated soluble domains, only a few atomic structures of these assembled molecular machines have been elucidated. To better understand the function and to solve the structure of protein complexes, it is thus necessary to design dedicated production and purification processes. Here we present cloning procedures to overproduce membrane proteins into Escherichia coli cells and describe the cloning and purification strategy for the Type VI secretion TssJLM membrane complex.

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Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 50%
Researcher 1 50%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 50%
Unknown 1 50%
Attention Score in Context

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This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 May 2018.
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#20,431,953
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