| Title |
A Combination of Stable Isotope Probing, Illumina Sequencing, and Co-occurrence Network to Investigate Thermophilic Acetate- and Lactate-Utilizing Bacteria
|
|---|---|
| Published in |
Microbial Ecology, July 2017
|
| DOI | 10.1007/s00248-017-1017-8 |
| Pubmed ID | |
| Authors |
Weimin Sun, Valdis Krumins, Yiran Dong, Pin Gao, Chunyan Ma, Min Hu, Baoqin Li, Bingqing Xia, Zijun He, Shangling Xiong |
| Abstract |
Anaerobic digestion is a complicated microbiological process that involves a wide diversity of microorganisms. Acetate is one of the most important intermediates, and interactions between acetate-oxidizing bacteria and archaea could play an important role in the formation of methane in anoxic environments. Anaerobic digestion at thermophilic temperatures is known to increase methane production, but the effects on the microbial community are largely unknown. In the current study, stable isotope probing was used to characterize acetate- and lactate-oxidizing bacteria in thermophilic anaerobic digestion. In microcosms fed (13)C-acetate, bacteria related to members of Clostridium, Hydrogenophaga, Fervidobacterium, Spirochaeta, Limnohabitans, and Rhodococcus demonstrated elevated abundances of (13)C-DNA fractions, suggesting their activities in acetate oxidation. In the treatments fed (13)C-lactate, Anaeromyxobacter, Desulfobulbus, Syntrophus, Cystobacterineae, and Azospira were found to be the potential thermophilic lactate utilizers. PICRUSt predicted that enzymes related to nitrate and nitrite reduction would be enriched in (13)C-DNA fractions, suggesting that the acetate and lactate oxidation may be coupled with nitrate and/or nitrite reduction. Co-occurrence network analysis indicated bacterial taxa not enriched in (13)C-DNA fractions that may also play a critical role in thermophilic anaerobic digestion. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 25% |
| Chile | 1 | 25% |
| Unknown | 2 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 44 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 7 | 16% |
| Student > Ph. D. Student | 6 | 14% |
| Student > Doctoral Student | 5 | 11% |
| Researcher | 5 | 11% |
| Student > Bachelor | 4 | 9% |
| Other | 7 | 16% |
| Unknown | 10 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 14 | 32% |
| Environmental Science | 6 | 14% |
| Biochemistry, Genetics and Molecular Biology | 4 | 9% |
| Engineering | 2 | 5% |
| Nursing and Health Professions | 1 | 2% |
| Other | 2 | 5% |
| Unknown | 15 | 34% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 January 2019.
All research outputs
#16,718,581
of 25,388,177 outputs
Outputs from Microbial Ecology
#1,490
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Outputs of similar age
#198,320
of 326,632 outputs
Outputs of similar age from Microbial Ecology
#40
of 52 outputs
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