| Title |
Quantification and comparison of acidic polysaccharides in edible fish intestines and livers using HPLC-MS/MS
|
|---|---|
| Published in |
Glycoconjugate Journal, July 2017
|
| DOI | 10.1007/s10719-017-9783-6 |
| Pubmed ID | |
| Authors |
Shuang Song, Qi Yu, Bao Zhang, Chunqing Ai, Yujiao Sun, Yinghuan Fu, Meiyu Zhao, Chengrong Wen |
| Abstract |
Fish intestines and livers are usually considered as delicious and nutritious food in China. Acidic polysaccharides are important nutrients in these food of animal origin, but there is currently little information regarding their quantitative distributions. The present study demonstrated a method to quantify acidic polysaccharides simultaneously by analyzing their disaccharides produced from the acid hydrolysis using high-performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometry. The recoveries for these acidic polysaccharides were all 97%-115% with relative standard deviation of 3.0%-9.0%. All of the acidic polysaccharides had good linearities. Then this method was applied to determine the composition of acidic polysaccharides in 5 edible fish livers and intestines. Besides well-known glycosaminoglycans (GAGs) including hyaluronic acid (HA), Chondroitin sulfate (CS), dermatan sulfate (DS) and heparin (HP), 4 novel acidic polysaccharides including 2 GAGs and 2 non-GAGs comprised of hexose-hexuronic acid repeating units were also found. CS and HP were the major acidic polysaccharides components in fish intestines and livers, respectively. The absolute amounts of acidic polysaccharides differed greatly in these fish tissues, but their proportions showed similarity in the same type of tissues. The present study demonstrated an effective method for acidic polysaccharides quantification, and revealed acidic polysaccharides compositions of edible fish livers and intestines. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 19% |
| Student > Doctoral Student | 2 | 10% |
| Student > Master | 2 | 10% |
| Researcher | 2 | 10% |
| Professor | 1 | 5% |
| Other | 3 | 14% |
| Unknown | 7 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 14% |
| Agricultural and Biological Sciences | 3 | 14% |
| Biochemistry, Genetics and Molecular Biology | 2 | 10% |
| Chemical Engineering | 1 | 5% |
| Economics, Econometrics and Finance | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 9 | 43% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 July 2017.
All research outputs
#22,764,772
of 25,382,440 outputs
Outputs from Glycoconjugate Journal
#870
of 929 outputs
Outputs of similar age
#285,559
of 326,157 outputs
Outputs of similar age from Glycoconjugate Journal
#9
of 11 outputs
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