| Title |
Acute mechanical overload increases IGF-I and MMP-9 mRNA in 3D tissue-engineered skeletal muscle
|
|---|---|
| Published in |
Biotechnology Techniques, February 2014
|
| DOI | 10.1007/s10529-014-1464-y |
| Pubmed ID | |
| Authors |
D. J. Player, N. R. W. Martin, S. L. Passey, A. P. Sharples, V. Mudera, M. P. Lewis |
| Abstract |
Skeletal muscle (SkM) is a tissue that responds to mechanical load following both physiological (exercise) or pathophysiological (bed rest) conditions. The heterogeneity of human samples and the experimental and ethical limitations of animal studies provide a rationale for the study of SkM plasticity in vitro. Many current in vitro approaches of mechanical loading of SkM disregard the three-dimensional (3D) structure in vivo. Tissue engineered 3D SkM, that displays highly aligned and differentiated myotubes, was used to investigate mechano-regulated gene transcription of genes implicated in hypertrophy/atrophy. Static loading (STL) and ramp loading (RPL) at 10 % strain for 60 min were used as mechano-stimulation with constructs sampled immediately for RNA extraction. STL increased IGF-I mRNA compared to both RPL and CON (control, p = 0.003 and 0.011 respectively) whilst MMP-9 mRNA increased in STL and RPL compared to CON (both p < 0.05). IGFBP-2 mRNA was differentially regulated in RPL and STL compared to CON (p = 0.057), whilst a reduction in IGFBP-5 mRNA was found for STL and RPL compared to CON (both p < 0.05). There was no effect in the expression of putative atrophic genes, myostatin, MuRF-1 and MAFBx (all p > 0.05). These data demonstrate a transcriptional signature associated with SkM hypertrophy within a tissue-engineered model that more greatly recapitulates the in vivo SkM structure compared previously published studies. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | 50% |
| United States | 2 | 33% |
| Norway | 1 | 17% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 50% |
| Scientists | 3 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 65 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 23 | 35% |
| Student > Master | 10 | 15% |
| Student > Bachelor | 7 | 11% |
| Researcher | 7 | 11% |
| Student > Doctoral Student | 4 | 6% |
| Other | 9 | 14% |
| Unknown | 5 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 12 | 18% |
| Agricultural and Biological Sciences | 8 | 12% |
| Medicine and Dentistry | 8 | 12% |
| Sports and Recreations | 7 | 11% |
| Engineering | 6 | 9% |
| Other | 13 | 20% |
| Unknown | 11 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 November 2014.
All research outputs
#8,534,528
of 25,373,627 outputs
Outputs from Biotechnology Techniques
#827
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Outputs of similar age
#81,011
of 239,845 outputs
Outputs of similar age from Biotechnology Techniques
#3
of 20 outputs
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So far Altmetric has tracked 2,762 research outputs from this source. They receive a mean Attention Score of 3.9. This one is in the 23rd percentile – i.e., 23% of its peers scored the same or lower than it.
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We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.