| Title |
A microdevice for parallelized pulmonary permeability studies
|
|---|---|
| Published in |
Biomedical Microdevices, December 2013
|
| DOI | 10.1007/s10544-013-9831-3 |
| Pubmed ID | |
| Authors |
Ludivine Bol, Jean-Christophe Galas, Hervé Hillaireau, Isabelle Le Potier, Valérie Nicolas, Anne-Marie Haghiri-Gosnet, Elias Fattal, Myriam Taverna |
| Abstract |
We describe a compartmentalized microdevice specifically designed to perform permeability studies across a model of lung barrier. Epithelial cell barriers were reproduced by culturing Calu-3 cells at the air-liquid interface (AIC) in 1 mm² microwells made from a perforated glass slide with an embedded porous membrane. We created a single basolateral reservoir for all microwells which eliminated the need to renew the growth medium during the culture growth phase. To perform drug permeability studies on confluent cell layers, the cell culture slide was aligned and joined to a collection platform consisting in 35 μL collection reservoirs connected at the top and bottom with microchannels. The integrity and functionality of the cell barriers were demonstrated by measurement of trans-epithelial electrical resistance (TEER), confocal imaging and permeability assays of ¹⁴C-sucrose. Micro-cell barriers were able to form confluent layers in 1 week, demonstrating a similar bioelectrical evolution as the Transwell systems used as controls. Tight junctions were observed throughout the cell-cell interfaces, and the low permeability coefficients of ¹⁴C-sucrose confirmed their functional presence, creating a primary barrier to the diffusion of solutes. This microdevice could facilitate the monitoring of biomolecule transport and the screening of formulations promoting their passage across the pulmonary barrier, in order to select candidates for pulmonary administration to patients. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 3% |
| Netherlands | 1 | 3% |
| Unknown | 37 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 23% |
| Student > Master | 8 | 21% |
| Researcher | 5 | 13% |
| Student > Bachelor | 4 | 10% |
| Professor | 2 | 5% |
| Other | 3 | 8% |
| Unknown | 8 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 8 | 21% |
| Biochemistry, Genetics and Molecular Biology | 5 | 13% |
| Medicine and Dentistry | 4 | 10% |
| Agricultural and Biological Sciences | 3 | 8% |
| Materials Science | 2 | 5% |
| Other | 8 | 21% |
| Unknown | 9 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 March 2014.
All research outputs
#14,620,730
of 23,404,576 outputs
Outputs from Biomedical Microdevices
#543
of 763 outputs
Outputs of similar age
#167,199
of 289,150 outputs
Outputs of similar age from Biomedical Microdevices
#3
of 4 outputs
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