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A Multi-Omics Analysis of Recombinant Protein Production in Hek293 Cells

Overview of attention for article published in PLOS ONE, August 2012
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  • Good Attention Score compared to outputs of the same age (74th percentile)
  • Good Attention Score compared to outputs of the same age and source (71st percentile)

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1 blog

Citations

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95 Dimensions

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275 Mendeley
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Title
A Multi-Omics Analysis of Recombinant Protein Production in Hek293 Cells
Published in
PLOS ONE, August 2012
DOI 10.1371/journal.pone.0043394
Pubmed ID
Authors

Stefanie Dietmair, Mark P. Hodson, Lake-Ee Quek, Nicholas E. Timmins, Peter Gray, Lars K. Nielsen

Abstract

Hek293 cells are the predominant hosts for transient expression of recombinant proteins and are used for stable expression of proteins where post-translational modifications performed by CHO cells are inadequate. Nevertheless, there is little information available on the key cellular features underpinning recombinant protein production in Hek293 cells. To improve our understanding of recombinant protein production in Hek293 cells and identify targets for the engineering of an improved host cell line, we have compared a stable, recombinant protein producing Hek293 cell line and its parental cell line using a combination of transcriptomics, metabolomics and fluxomics. Producer cultures consumed less glucose than non-producer cultures while achieving the same growth rate, despite the additional burden of recombinant protein production. Surprisingly, there was no indication that producer cultures compensated for the reduction in glycolytic energy by increasing the efficiency of glucose utilization or increasing glutamine consumption. In contrast, glutamine consumption was lower and the majority of genes involved in oxidative phosphorylation were downregulated in producer cultures. We observed an overall downregulation of a large number of genes associated with broad cellular functions (e.g., cell growth and proliferation) in producer cultures, and therefore speculate that a broad adaptation of the cellular network freed up resources for recombinant protein production while maintaining the same growth rate. Increased abundance of genes associated with endoplasmic reticulum stress indicated a possible bottleneck at the point of protein folding and assembly.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 275 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Italy 2 <1%
Chile 1 <1%
Germany 1 <1%
India 1 <1%
Canada 1 <1%
Denmark 1 <1%
Spain 1 <1%
United States 1 <1%
Unknown 266 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 61 22%
Student > Ph. D. Student 59 21%
Student > Master 33 12%
Student > Bachelor 29 11%
Student > Doctoral Student 16 6%
Other 29 11%
Unknown 48 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 92 33%
Biochemistry, Genetics and Molecular Biology 65 24%
Engineering 15 5%
Chemical Engineering 15 5%
Chemistry 8 3%
Other 27 10%
Unknown 53 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 October 2015.
All research outputs
#5,870,000
of 22,747,498 outputs
Outputs from PLOS ONE
#70,517
of 194,162 outputs
Outputs of similar age
#42,045
of 169,410 outputs
Outputs of similar age from PLOS ONE
#1,232
of 4,365 outputs
Altmetric has tracked 22,747,498 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 194,162 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.1. This one has gotten more attention than average, scoring higher than 63% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 169,410 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 4,365 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.