| Title |
Microchip electrophoresis with laser-induced fluorescence detection for the determination of the ratio of nitric oxide to superoxide production in macrophages during inflammation
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, May 2017
|
| DOI | 10.1007/s00216-017-0401-z |
| Pubmed ID | |
| Authors |
Giuseppe Caruso, Claudia G. Fresta, Joseph M. Siegel, Manjula B. Wijesinghe, Susan M. Lunte |
| Abstract |
It is well known that excessive production of reactive oxygen and nitrogen species is linked to the development of oxidative stress-driven disorders. In particular, nitric oxide (NO) and superoxide (O2(•-)) play critical roles in many physiological and pathological processes. This article reports the use of 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate and MitoSOX Red in conjunction with microchip electrophoresis and laser-induced fluorescence detection for the simultaneous detection of NO and O2(•-) in RAW 264.7 macrophage cell lysates following different stimulation procedures. Cell stimulations were performed in the presence and absence of cytosolic (diethyldithiocarbamate) and mitochondrial (2-methoxyestradiol) superoxide dismutase (SOD) inhibitors. The NO/O2(•-) ratios in macrophage cell lysates under physiological and proinflammatory conditions were determined. The NO/O2(•-) ratios were 0.60 ± 0.07 for unstimulated cells pretreated with SOD inhibitors, 1.08 ± 0.06 for unstimulated cells in the absence of SOD inhibitors, and 3.14 ± 0.13 for stimulated cells. The effect of carnosine (antioxidant) or Ca(2+) (intracellular messenger) on the NO/O2(•-) ratio was also investigated. Graphical Abstract Simultaneous detection of nitric oxide and superoxide in macrophage cell lysates. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 7% |
| Unknown | 14 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 27% |
| Researcher | 4 | 27% |
| Student > Bachelor | 2 | 13% |
| Student > Ph. D. Student | 1 | 7% |
| Other | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 2 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 6 | 40% |
| Nursing and Health Professions | 1 | 7% |
| Medicine and Dentistry | 1 | 7% |
| Agricultural and Biological Sciences | 1 | 7% |
| Neuroscience | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 4 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 July 2017.
All research outputs
#19,951,180
of 25,382,440 outputs
Outputs from Analytical & Bioanalytical Chemistry
#6,061
of 9,619 outputs
Outputs of similar age
#237,344
of 327,782 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#51
of 139 outputs
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