Title |
Conducting Polymer Electrodes for Gel Electrophoresis
|
---|---|
Published in |
PLOS ONE, February 2014
|
DOI | 10.1371/journal.pone.0089416 |
Pubmed ID | |
Authors |
Katarina Bengtsson, Sara Nilsson, Nathaniel D. Robinson |
Abstract |
In nearly all cases, electrophoresis in gels is driven via the electrolysis of water at the electrodes, where the process consumes water and produces electrochemical by-products. We have previously demonstrated that π-conjugated polymers such as poly(3,4-ethylenedioxythiophene) (PEDOT) can be placed between traditional metal electrodes and an electrolyte to mitigate electrolysis in liquid (capillary electroosmosis/electrophoresis) systems. In this report, we extend our previous result to gel electrophoresis, and show that electrodes containing PEDOT can be used with a commercial polyacrylamide gel electrophoresis system with minimal impact to the resulting gel image or the ionic transport measured during a separation. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 58 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 12 | 21% |
Student > Master | 9 | 16% |
Researcher | 9 | 16% |
Student > Bachelor | 6 | 10% |
Student > Doctoral Student | 4 | 7% |
Other | 11 | 19% |
Unknown | 7 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 13 | 22% |
Biochemistry, Genetics and Molecular Biology | 9 | 16% |
Chemistry | 8 | 14% |
Agricultural and Biological Sciences | 8 | 14% |
Materials Science | 5 | 9% |
Other | 7 | 12% |
Unknown | 8 | 14% |