Title |
食中毒を引き起こす有毒キノコの種特異的プライマーによるスクリーニング法の開発
|
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Published in |
Shokuhin eiseigaku zasshi Journal of the Food Hygienic Society of Japan, June 2017
|
DOI | 10.3358/shokueishi.58.132 |
Pubmed ID | |
Authors |
Chie Nomura, Atsushi Masayama, Mizuka Yamaguchi, Daisuke Sakuma, Keiji Kajimura |
Abstract |
In this study, species-specific identification of five toxic mushrooms, Chlorophyllum molybdites, Gymnopilus junonius, Hypholoma fasciculare, Pleurocybella porrigens, and Tricholoma ustale, which have been involved in food-poisoning incidents in Japan, was investigated. Specific primer pairs targeting internal transcribed spacer (ITS) regions were designed for PCR detection. The specific amplicons were obtained from fresh, cooked, and simulated gastric fluid (SGF)-treated samples. No amplicons were detected from other mushrooms with similar morphology. Our method using one-step extraction of mushrooms allows rapid detection within 2.5 hr. It could be utilized for rapid identification or screening of toxic mushrooms. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 2 | 67% |
Spain | 1 | 33% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 25% |
Student > Master | 2 | 17% |
Student > Bachelor | 2 | 17% |
Unspecified | 1 | 8% |
Unknown | 4 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 17% |
Agricultural and Biological Sciences | 2 | 17% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
Unspecified | 1 | 8% |
Nursing and Health Professions | 1 | 8% |
Other | 1 | 8% |
Unknown | 4 | 33% |