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Dry-Coated Live Viral Vector Vaccines Delivered by Nanopatch Microprojections Retain Long-Term Thermostability and Induce Transgene-Specific T Cell Responses in Mice

Overview of attention for article published in PLOS ONE, July 2013
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (95th percentile)
  • High Attention Score compared to outputs of the same age and source (92nd percentile)

Mentioned by

news
2 news outlets
blogs
3 blogs
patent
2 patents

Citations

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69 Dimensions

Readers on

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86 Mendeley
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Title
Dry-Coated Live Viral Vector Vaccines Delivered by Nanopatch Microprojections Retain Long-Term Thermostability and Induce Transgene-Specific T Cell Responses in Mice
Published in
PLOS ONE, July 2013
DOI 10.1371/journal.pone.0067888
Pubmed ID
Authors

Frances E. Pearson, Celia L. McNeilly, Michael L. Crichton, Clare A. Primiero, Sally R. Yukiko, Germain J. P. Fernando, Xianfeng Chen, Sarah C. Gilbert, Adrian V. S. Hill, Mark A. F. Kendall

Abstract

The disadvantages of needle-based immunisation motivate the development of simple, low cost, needle-free alternatives. Vaccine delivery to cutaneous environments rich in specialised antigen-presenting cells using microprojection patches has practical and immunological advantages over conventional needle delivery. Additionally, stable coating of vaccine onto microprojections removes logistical obstacles presented by the strict requirement for cold-chain storage and distribution of liquid vaccine, or lyophilised vaccine plus diluent. These attributes make these technologies particularly suitable for delivery of vaccines against diseases such as malaria, which exerts its worst effects in countries with poorly-resourced healthcare systems. Live viral vectors including adenoviruses and poxviruses encoding exogenous antigens have shown significant clinical promise as vaccines, due to their ability to generate high numbers of antigen-specific T cells. Here, the simian adenovirus serotype 63 and the poxvirus modified vaccinia Ankara--two vectors under evaluation for the delivery of malaria antigens to humans--were formulated for coating onto Nanopatch microprojections and applied to murine skin. Co-formulation with the stabilising disaccharides trehalose and sucrose protected virions during the dry-coating process. Transgene-specific CD8(+) T cell responses following Nanopatch delivery of both vectors were similar to intradermal injection controls after a single immunisation (despite a much lower delivered dose), though MVA boosting of pre-primed responses with Nanopatch was found to be less effective than the ID route. Importantly, disaccharide-stabilised ChAd63 could be stored for 10 weeks at 37°C with less than 1 log10 loss of viability, and retained single-dose immunogenicity after storage. These data support the further development of microprojection patches for the deployment of live vaccines in hot climates.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 86 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 1%
United States 1 1%
Australia 1 1%
Unknown 83 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 21 24%
Student > Bachelor 13 15%
Student > Ph. D. Student 11 13%
Student > Master 11 13%
Student > Postgraduate 6 7%
Other 17 20%
Unknown 7 8%
Readers by discipline Count As %
Medicine and Dentistry 19 22%
Agricultural and Biological Sciences 17 20%
Biochemistry, Genetics and Molecular Biology 12 14%
Engineering 7 8%
Immunology and Microbiology 6 7%
Other 14 16%
Unknown 11 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 38. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 July 2020.
All research outputs
#901,197
of 22,747,498 outputs
Outputs from PLOS ONE
#12,311
of 194,162 outputs
Outputs of similar age
#7,966
of 194,284 outputs
Outputs of similar age from PLOS ONE
#357
of 4,752 outputs
Altmetric has tracked 22,747,498 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 194,162 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.1. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 194,284 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 4,752 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.