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2D PAGE: Sample Preparation and Fractionation

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Cover of '2D PAGE: Sample Preparation and Fractionation'

Table of Contents

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    Book Overview
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    Chapter 1 Mechanical/physical methods of cell disruption and tissue homogenization.
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    Chapter 2 Bacteria and Yeast Cell Disruption Using Lytic Enzymes
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    Chapter 3 Sample Solublization Buffers for Two-Dimensional Electrophoresis
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    Chapter 4 Quantitation of Protein in Samples Prepared for 2-D Electrophoresis
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    Chapter 5 Removal of Interfering Substances in Samples Prepared for Two-Dimensional (2-D) Electrophoresis
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    Chapter 6 Protein Concentration by Hydrophilic Interaction Chromatography Combined with Solid Phase Extraction
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    Chapter 7 Difference Gel Electrophoresis Based on Lys/Cys Tagging
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    Chapter 8 Isotope-Coded Two-Dimensional Maps: Tagging with Deuterated Acrylamide and 2-Vinylpyridine
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    Chapter 9 Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
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    Chapter 10 ICPL—Isotope-Coded Protein Label
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    Chapter 11 Radiolabeling for Two-Dimensional Gel Analysis
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    Chapter 12 Sequential Extraction of Proteins by Chemical Reagents
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    Chapter 13 Reducing Sample Complexity by RP-HPLC: Beyond the Tip of the Protein Expression Iceberg
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    Chapter 14 Enriching Basic and Acidic Rat Brain Proteins with Ion Exchange Mini Spin Columns Before Two-Dimensional Gel Electrophoresis
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    Chapter 15 Reducing the Complexity of the Escherichia coli Proteome by Chromatography on Reactive Dye Columns
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    Chapter 16 Reducing Sample Complexity in Proteomics by Chromatofocusing with Simple Buffer Mixtures
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    Chapter 17 Fractionation of Proteins by Immobilized Metal Affinity Chromatography
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    Chapter 18 Fractionation of Proteins by Heparin Chromatography
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    Chapter 19 Fractionation of complex protein mixtures by liquid-phase isoelectric focusing.
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    Chapter 20 Microscale Isoelectric Focusing in Solution
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    Chapter 21 2D PAGE: Sample Preparation and Fractionation
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    Chapter 22 Sample Prefractionation in Granulated Sephadex IEF Gels
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    Chapter 23 Free-Flow Electrophoresis System for Plasma Proteomic Applications
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    Chapter 24 Protein Fractionation by Preparative Electrophoresis
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    Chapter 25 2D PAGE: Sample Preparation and Fractionation
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    Chapter 26 Isolation of Highly Pure Rat Liver Mitochondria with the Aid of Zone-Electrophoresis in a Free Flow Device (ZE-FFE)
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    Chapter 27 Isolation of Proteins and Protein Complexes by Immunoprecipitation
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    Chapter 28 Isolation of Phosphoproteins
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    Chapter 29 Glycoprotein Enrichment Through Lectin Affinity Techniques
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    Chapter 30 Isolation of bacterial cell membranes proteins using carbonate extraction.
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    Chapter 31 Enrichment of Membrane Proteins by Partitioning in Detergent/Polymer Aqueous Two-Phase Systems
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    Chapter 32 The Isolation of Detergent-Resistant Lipid Rafts for Two-Dimensional Electrophoresis
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    Chapter 33 Isolation of Membrane Protein Complexes by Blue Native Electrophoresis
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    Chapter 34 Tissue Microdissection
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Title
2D PAGE: Sample Preparation and Fractionation
Published by
Humana Press, January 2008
DOI 10.1007/978-1-60327-064-9
ISBNs
978-1-58829-722-8, 978-1-60327-064-9
Authors

Posch, Andrew

Editors

Anton Posch

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 114 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 2 2%
India 1 <1%
Malaysia 1 <1%
Peru 1 <1%
United States 1 <1%
Unknown 108 95%

Demographic breakdown

Readers by professional status Count As %
Researcher 21 18%
Student > Bachelor 21 18%
Student > Ph. D. Student 20 18%
Student > Master 20 18%
Student > Doctoral Student 7 6%
Other 13 11%
Unknown 12 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 31 27%
Biochemistry, Genetics and Molecular Biology 18 16%
Chemistry 15 13%
Engineering 8 7%
Medicine and Dentistry 6 5%
Other 17 15%
Unknown 19 17%