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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase
|
|---|---|
| Published in |
Science, August 1990
|
| DOI | 10.1126/science.2200121 |
| Pubmed ID | |
| Authors |
Craig Tuerk, Larry Gold |
| Abstract |
High-affinity nucleic acid ligands for a protein were isolated by a procedure that depends on alternate cycles of ligand selection from pools of variant sequences and amplification of the bound species. Multiple rounds exponentially enrich the population for the highest affinity species that can be clonally isolated and characterized. In particular one eight-base region of an RNA that interacts with the T4 DNA polymerase was chosen and randomized. Two different sequences were selected by this procedure from the calculated pool of 65,536 species. One is the wild-type sequence found in the bacteriophage mRNA; one is varied from wild type at four positions. The binding constants of these two RNA's to T4 DNA polymerase are equivalent. These protocols with minimal modification can yield high-affinity ligands for any protein that binds nucleic acids as part of its function; high-affinity ligands could conceivably be developed for any target molecule. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 3,363 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 42 | 1% |
| Germany | 10 | <1% |
| United Kingdom | 9 | <1% |
| France | 5 | <1% |
| South Africa | 5 | <1% |
| Canada | 5 | <1% |
| Austria | 4 | <1% |
| Japan | 3 | <1% |
| Turkey | 2 | <1% |
| Other | 23 | <1% |
| Unknown | 3255 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 879 | 26% |
| Student > Master | 466 | 14% |
| Researcher | 426 | 13% |
| Student > Bachelor | 388 | 12% |
| Student > Doctoral Student | 162 | 5% |
| Other | 392 | 12% |
| Unknown | 650 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 692 | 21% |
| Biochemistry, Genetics and Molecular Biology | 691 | 21% |
| Chemistry | 586 | 17% |
| Engineering | 186 | 6% |
| Medicine and Dentistry | 81 | 2% |
| Other | 391 | 12% |
| Unknown | 736 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 69. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 March 2024.
All research outputs
#626,755
of 26,017,215 outputs
Outputs from Science
#13,952
of 83,593 outputs
Outputs of similar age
#52
of 14,766 outputs
Outputs of similar age from Science
#3
of 165 outputs
Altmetric has tracked 26,017,215 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 83,593 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 65.8. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 14,766 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 165 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.