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Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase

Overview of attention for article published in Science, August 1990
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (98th percentile)

Mentioned by

news
1 news outlet
blogs
5 blogs
policy
3 policy sources
twitter
1 X user
patent
713 patents
facebook
1 Facebook page
wikipedia
13 Wikipedia pages

Citations

dimensions_citation
8786 Dimensions

Readers on

mendeley
3364 Mendeley
citeulike
16 CiteULike
connotea
1 Connotea
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Title
Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase
Published in
Science, August 1990
DOI 10.1126/science.2200121
Pubmed ID
Authors

Craig Tuerk, Larry Gold

Abstract

High-affinity nucleic acid ligands for a protein were isolated by a procedure that depends on alternate cycles of ligand selection from pools of variant sequences and amplification of the bound species. Multiple rounds exponentially enrich the population for the highest affinity species that can be clonally isolated and characterized. In particular one eight-base region of an RNA that interacts with the T4 DNA polymerase was chosen and randomized. Two different sequences were selected by this procedure from the calculated pool of 65,536 species. One is the wild-type sequence found in the bacteriophage mRNA; one is varied from wild type at four positions. The binding constants of these two RNA's to T4 DNA polymerase are equivalent. These protocols with minimal modification can yield high-affinity ligands for any protein that binds nucleic acids as part of its function; high-affinity ligands could conceivably be developed for any target molecule.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3,364 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 42 1%
Germany 10 <1%
United Kingdom 9 <1%
France 5 <1%
South Africa 5 <1%
Canada 5 <1%
Austria 4 <1%
Japan 3 <1%
Turkey 2 <1%
Other 23 <1%
Unknown 3256 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 881 26%
Student > Master 466 14%
Researcher 434 13%
Student > Bachelor 383 11%
Student > Doctoral Student 165 5%
Other 395 12%
Unknown 640 19%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 692 21%
Agricultural and Biological Sciences 690 21%
Chemistry 592 18%
Engineering 185 5%
Medicine and Dentistry 84 2%
Other 395 12%
Unknown 726 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 69. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 February 2024.
All research outputs
#590,866
of 24,727,020 outputs
Outputs from Science
#12,868
of 80,074 outputs
Outputs of similar age
#54
of 14,742 outputs
Outputs of similar age from Science
#3
of 163 outputs
Altmetric has tracked 24,727,020 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 80,074 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 64.7. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 14,742 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 163 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.