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Mendeley readers
Attention Score in Context
| Title |
Antiprion compounds that reduce PrPSc levels in dividing and stationary-phase cells
|
|---|---|
| Published in |
Bioorganic & Medicinal Chemistry, September 2013
|
| DOI | 10.1016/j.bmc.2013.09.022 |
| Pubmed ID | |
| Authors |
B. Michael Silber, Joel R. Gever, Zhe Li, Alejandra Gallardo-Godoy, Adam R. Renslo, Kartika Widjaja, John J. Irwin, Satish Rao, Matthew P. Jacobson, Sina Ghaemmaghami, Stanley B. Prusiner |
| Abstract |
During prion diseases, a normally benign, host protein, denoted PrP(C), undergoes alternative folding into the aberrant isoform, PrP(Sc). We used ELISA to identify and confirm hits in order to develop leads that reduce PrP(Sc) in prion-infected dividing and stationary-phase mouse neuroblastoma (ScN2a-cl3) cells. We tested 52,830 diverse small molecules in dividing cells and 49,430 in stationary-phase cells. This led to 3100 HTS and 970 single point confirmed (SPC) hits in dividing cells, 331 HTS and 55 confirmed SPC hits in stationary-phase cells as well as 36 confirmed SPC hits active in both. Fourteen chemical leads were identified from confirmed SPC hits in dividing cells and three in stationary-phase cells. From more than 682 compounds tested in concentration-effect relationships in dividing cells to determine potency (EC50), 102 had EC50 values between 1 and 10 μM and 50 had EC50 values of <1 μM; none affected cell viability. We observed an excellent correlation between EC50 values determined by ELISA and Western immunoblotting for 28 representative compounds in dividing cells (R(2)=0.75; p <0.0001). Of the 55 confirmed SPC hits in stationary-phase cells, 23 were piperazine, indole, or urea leads. The EC50 values of one indole in stationary-phase and dividing ScN2a-cl3 cells were 7.5 and 1.6 μM, respectively. Unexpectedly, the number of hits in stationary-phase cells was ~10% of that in dividing cells. The explanation for this difference remains to be determined. |
Mendeley readers
The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 33 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 6 | 18% |
| Student > Ph. D. Student | 5 | 15% |
| Researcher | 4 | 12% |
| Professor > Associate Professor | 4 | 12% |
| Student > Master | 4 | 12% |
| Other | 5 | 15% |
| Unknown | 5 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 21% |
| Chemistry | 6 | 18% |
| Agricultural and Biological Sciences | 4 | 12% |
| Medicine and Dentistry | 3 | 9% |
| Neuroscience | 3 | 9% |
| Other | 4 | 12% |
| Unknown | 6 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 March 2022.
All research outputs
#3,138,276
of 25,371,288 outputs
Outputs from Bioorganic & Medicinal Chemistry
#385
of 7,493 outputs
Outputs of similar age
#26,980
of 213,362 outputs
Outputs of similar age from Bioorganic & Medicinal Chemistry
#2
of 65 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 7,493 research outputs from this source. They receive a mean Attention Score of 4.8. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 213,362 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 65 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.