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A new visually improved and sensitive loop mediated isothermal amplification (LAMP) for diagnosis of symptomatic falciparum malaria

Overview of attention for article published in Acta Tropica, March 2014
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (66th percentile)
  • High Attention Score compared to outputs of the same age and source (86th percentile)

Mentioned by

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1 patent
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1 Facebook page

Citations

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42 Dimensions

Readers on

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91 Mendeley
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Title
A new visually improved and sensitive loop mediated isothermal amplification (LAMP) for diagnosis of symptomatic falciparum malaria
Published in
Acta Tropica, March 2014
DOI 10.1016/j.actatropica.2014.02.016
Pubmed ID
Authors

Abu Naser Mohon, Rubayet Elahi, Wasif A Khan, Rashidul Haque, David J Sullivan, Mohammad Shafiul Alam

Abstract

Molecular diagnosis of malaria by nucleotide amplification requires sophisticated and expensive instruments, typically found only in well-established laboratories. Loop-mediated isothermal amplification (LAMP) has provided a new platform for an easily adaptable molecular technique for molecular diagnosis of malaria without the use of expensive instruments. A new primer set has been designed targeting the 18S rRNA gene for the detection of Plasmodium falciparum in whole blood samples. The efficacy of LAMP using the new primer set was assessed in this study in comparison to that of a previously described set of LAMP primers as well as with microscopy and real-time PCR as reference methods for detecting P. falciparum. Pre-addition of hydroxy napthol blue (HNB) in the LAMP reaction caused a distinct color change, thereby improving the visual detection system. The new LAMP assay was found to be 99.1% sensitive compared to microscopy and 98.1% when compared to real-time PCR. Meanwhile, its specificity was 99% and 100% in contrast to microscopy and real-time PCR, respectively. Moreover, the LAMP method was in very good agreement with microscopy and real-time PCR (0.94 and 0.98, respectively). This new LAMP method can detect at least 5parasites/μL of infected blood within 35min, while the other LAMP method tested in this study, could detect a minimum of 100parasites/μL of human blood after 60min of amplification. Thus, the new method is sensitive and specific, can be carried out in a very short time, and can substitute PCR in healthcare clinics and standard laboratories.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 91 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Burkina Faso 1 1%
Switzerland 1 1%
Tanzania, United Republic of 1 1%
United Kingdom 1 1%
Spain 1 1%
Poland 1 1%
Unknown 85 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 23 25%
Researcher 15 16%
Student > Master 14 15%
Student > Bachelor 7 8%
Student > Postgraduate 6 7%
Other 15 16%
Unknown 11 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 24 26%
Biochemistry, Genetics and Molecular Biology 17 19%
Medicine and Dentistry 11 12%
Immunology and Microbiology 5 5%
Engineering 4 4%
Other 12 13%
Unknown 18 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 June 2022.
All research outputs
#8,262,445
of 25,374,917 outputs
Outputs from Acta Tropica
#759
of 3,515 outputs
Outputs of similar age
#76,054
of 235,875 outputs
Outputs of similar age from Acta Tropica
#5
of 44 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one has received more attention than most of these and is in the 66th percentile.
So far Altmetric has tracked 3,515 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done well, scoring higher than 77% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 235,875 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 44 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.