| Title |
Direct analysis of site-specific N-glycopeptides of serological proteins in dried blood spot samples
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, July 2017
|
| DOI | 10.1007/s00216-017-0438-z |
| Pubmed ID | |
| Authors |
Na Young Choi, Heeyoun Hwang, Eun Sun Ji, Gun Wook Park, Ju Yeon Lee, Hyun Kyoung Lee, Jin Young Kim, Jong Shin Yoo |
| Abstract |
Dried blood spot (DBS) samples have a number of advantages, especially with respect to ease of collection, transportation, and storage and to reduce biohazard risk. N-glycosylation is a major post-translational modification of proteins in human blood that is related to a variety of biological functions, including metastasis, cell-cell interactions, inflammation, and immunization. Here, we directly analyzed tryptic N-glycopeptides from glycoproteins in DBS samples using liquid chromatography-tandem mass spectrometry (LC-MS/MS) without centrifugation of blood samples, depletion of major proteins, desalting of tryptic peptides, and enrichment of N-glycopeptides. Using this simple method, we identified a total of 41 site-specific N-glycopeptides from 16 glycoproteins in the DBS samples, from immunoglobulin gamma 1 (IgG-1, 10 mg/mL) down to complement component C7 (50 μg/mL). Of these, 32 N-glycopeptides from 14 glycoproteins were consistently quantified over 180 days stored at room temperature. The major abundant glycoproteins in the DBS samples were IgG-1 and IgG-2, which contain nine asialo-fucosylated complex types of 16 different N-glycopeptide isoforms. Sialo-non-fucosylated complex types were primarily detected in the other glycoproteins such as alpha-1-acid glycoprotein 1, 2, alpha-1-antitypsin, alpha-2-macroglobulin, haptoglobin, hemopexin, Ig alpha 1, 2 chain C region, kininogen-1, prothrombin, and serotransferrin. We first report the characterization of site-specific N-glycoproteins in DBS samples by LC-MS/MS with minimal sample preparation. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 67% |
| Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 25 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 24% |
| Student > Master | 4 | 16% |
| Researcher | 4 | 16% |
| Student > Bachelor | 2 | 8% |
| Other | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 6 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 6 | 24% |
| Biochemistry, Genetics and Molecular Biology | 4 | 16% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 8% |
| Medicine and Dentistry | 2 | 8% |
| Agricultural and Biological Sciences | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 9 | 36% |
Attention Score in Context
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#19,951,180
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Outputs from Analytical & Bioanalytical Chemistry
#6,061
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#236,862
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Outputs of similar age from Analytical & Bioanalytical Chemistry
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