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Mendeley readers
Attention Score in Context
| Title |
Genome‐wide DNA methylation analysis of formalin‐fixed paraffin embedded colorectal cancer tissue
|
|---|---|
| Published in |
Genes, Chromosomes, and Cancer, March 2014
|
| DOI | 10.1002/gcc.22164 |
| Pubmed ID | |
| Authors |
Troy D. Dumenil, Leesa F. Wockner, Mark Bettington, Diane M. McKeone, Kerenaftali Klein, Lisa M. Bowdler, Grant W. Montgomery, Barbara A. Leggett, Vicki L.J. Whitehall |
| Abstract |
Formalin fixation and embedding of clinical tissue samples in paraffin is a common method for archiving biological material. These samples are often well annotated and provide an invaluable resource for research. However, this process of fixation and storage of tissue leads to DNA damage and fragmentation. The use of DNA from formalin fixed, paraffin-embedded (FFPE) tissue to interrogate methylation levels on a genome-wide scale can pose challenges. We compared fresh and matched FFPE tissue DNA samples using the Illumina Infinium HD Human Methylation 450K BeadChip platform with a companion application for repair and "restoration" of DNA from FFPE tissue. Our results showed good correlation between fresh and FFPE sample data. FFPE DNA captured 99% of the CpG sites on the array on average. Significant cancer subgroups based on the CpG island methylator phenotype (CIMP) were clearly distinguished for both fresh and FFPE sample sets with cluster and scaling analysis. The DNA methylation status for the five standard CIMP panel genes which was evaluated for all samples by the MethyLight assay was correctly assigned in both fresh and FFPE samples by the array data. We conclude that the "restoration" method followed by assay on the Infinium HD Human Methylation 450K microarray can produce good quality data for DNA from FFPE samples. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 2% |
| Hong Kong | 1 | 2% |
| Denmark | 1 | 2% |
| Canada | 1 | 2% |
| Unknown | 48 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 14 | 27% |
| Student > Master | 8 | 15% |
| Student > Bachelor | 6 | 12% |
| Researcher | 6 | 12% |
| Professor | 4 | 8% |
| Other | 10 | 19% |
| Unknown | 4 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 17 | 33% |
| Medicine and Dentistry | 13 | 25% |
| Agricultural and Biological Sciences | 9 | 17% |
| Neuroscience | 2 | 4% |
| Business, Management and Accounting | 2 | 4% |
| Other | 3 | 6% |
| Unknown | 6 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 October 2015.
All research outputs
#20,657,128
of 25,374,917 outputs
Outputs from Genes, Chromosomes, and Cancer
#1,307
of 1,541 outputs
Outputs of similar age
#175,363
of 238,319 outputs
Outputs of similar age from Genes, Chromosomes, and Cancer
#8
of 14 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,541 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 8th percentile – i.e., 8% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 238,319 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one is in the 7th percentile – i.e., 7% of its contemporaries scored the same or lower than it.