Title |
Worldwide dissemination of acquired carbapenem-hydrolysing class D β-lactamases in Acinetobacter spp. other than Acinetobacter baumannii
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Published in |
International Journal of Antimicrobial Agents, February 2014
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DOI | 10.1016/j.ijantimicag.2014.01.012 |
Pubmed ID | |
Authors |
Esther Zander, Ana Fernández-González, Xenia Schleicher, Cathrin Dammhayn, Witchuda Kamolvit, Harald Seifert, Paul G. Higgins |
Abstract |
The aim of this study was to identify acquired OXA-type carbapenemases in Acinetobacter spp. other than Acinetobacter baumannii. From a total of 453 carbapenem-susceptible and -resistant Acinetobacter isolates collected worldwide, 23 were positive for blaOXA genes by multiplex PCR. These isolates were identified as Acinetobacter pittii (n=18), Acinetobacter nosocomialis (n=2), Acinetobacter junii (n=1) and Acinetobacter genomic species 14TU/13BJ (n=2). The blaOXA genes and associated insertion sequence (IS) elements were sequenced by primer walking. In 11 of these isolates, sequencing of the PCR products revealed that they were false-positive for blaOXA. The remaining 12 isolates, originating from Europe, Asia, South America, North America and South Africa, harboured OXA-23 (n=4), OXA-58 (n=5), OXA-40-like (n=1) and OXA-143-like (n=1); one A. pittii isolate harboured both OXA-23 and OXA-58. IS elements were associated with blaOXA in 10 isolates. OXA multiplex PCR showed a high degree of false-positive results (47.8%), indicating that detection of blaOXA in non-baumanniiAcinetobacter spp. should be confirmed using additional methods. |
X Demographics
Geographical breakdown
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United States | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Portugal | 1 | 2% |
Brazil | 1 | 2% |
Unknown | 56 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 12 | 21% |
Student > Ph. D. Student | 9 | 16% |
Student > Master | 8 | 14% |
Student > Doctoral Student | 4 | 7% |
Student > Bachelor | 4 | 7% |
Other | 9 | 16% |
Unknown | 12 | 21% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 10 | 17% |
Biochemistry, Genetics and Molecular Biology | 10 | 17% |
Medicine and Dentistry | 8 | 14% |
Immunology and Microbiology | 7 | 12% |
Environmental Science | 2 | 3% |
Other | 4 | 7% |
Unknown | 17 | 29% |