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A degenerate PCR-based strategy as a means of identifying homologues of aminoglycoside and β-lactam resistance genes in the gut microbiota

Overview of attention for article published in BMC Microbiology, February 2014
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Title
A degenerate PCR-based strategy as a means of identifying homologues of aminoglycoside and β-lactam resistance genes in the gut microbiota
Published in
BMC Microbiology, February 2014
DOI 10.1186/1471-2180-14-25
Pubmed ID
Authors

Fiona Fouhy, R Paul Ross, Gerald F Fitzgerald, Catherine Stanton, Paul D Cotter

Abstract

The potential for the human gut microbiota to serve as a reservoir for antibiotic resistance genes has been the subject of recent discussion. However, this has yet to be investigated using a rapid PCR-based approach. In light of this, here we aim to determine if degenerate PCR primers can detect aminoglycoside and β-lactam resistance genes in the gut microbiota of healthy adults, without the need for an initial culture-based screen for resistant isolates. In doing so, we would determine if the gut microbiota of healthy adults, lacking recent antibiotic exposure, is a reservoir for resistance genes.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 65 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 13 20%
Researcher 9 14%
Student > Master 7 11%
Student > Bachelor 7 11%
Other 3 5%
Other 10 15%
Unknown 16 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 14 22%
Biochemistry, Genetics and Molecular Biology 10 15%
Immunology and Microbiology 7 11%
Medicine and Dentistry 5 8%
Environmental Science 4 6%
Other 6 9%
Unknown 19 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 February 2014.
All research outputs
#18,369,403
of 22,751,628 outputs
Outputs from BMC Microbiology
#2,234
of 3,180 outputs
Outputs of similar age
#229,412
of 307,220 outputs
Outputs of similar age from BMC Microbiology
#59
of 77 outputs
Altmetric has tracked 22,751,628 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,180 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 15th percentile – i.e., 15% of its peers scored the same or lower than it.
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We're also able to compare this research output to 77 others from the same source and published within six weeks on either side of this one. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.