| Title |
Selective Cytotoxicity of a Red Grape Wine Flavonoid Fraction Against MCF-7 Cells
|
|---|---|
| Published in |
Breast Cancer Research and Treatment, May 2004
|
| DOI | 10.1023/b:brea.0000021048.52430.c0 |
| Pubmed ID | |
| Authors |
Fatima Hakimuddin, Gopinadhan Paliyath, Kelly Meckling |
| Abstract |
Red wine is a rich source of polyphenolic components such as anthocyanins and flavonoids. The inhibitory effects of red wine polyphenolics on human breast cancer cells have been demonstrated earlier, but their effects on normal cells have not been fully established. Red wine (Merlot) was fractionated by hydrophobic interaction chromatography and different flavonoid fractions with increasing hydrophobicity were obtained. These fractions were tested for their inhibitory effect on human breast cancer cells (MCF-7), normal human mammary epithelial cells (HMEC), and a non-tumorigenic MCF-10A cell line. By contrast to the authentic flavonoids such as quercetin, naringenin and catechin which inhibited the growth of HMEC much more than that of MCF-7 cancer cells, a red wine fraction, that was comprised mainly of the flavonoid aglycones, showed maximal inhibition of the growth of breast cancer cells, with relatively low cytotoxicity towards HMEC and MCF-10A cells. In the presence of this flavonoid fraction, the normal cells grew normally, whereas the breast cancer cells underwent a change in morphology into spherical forms. Cytotoxicity analyses suggested that these cells had become apoptotic. The efficiency of inhibition of cell proliferation by various flavonoid fractions appeared to be related to their inhibition of calcium and calmodulin-promoted phosphodiesterase activity, suggesting that flavonoids may interfere with calcium second messenger function. The results suggest that certain grape wine ingredients have anticancer properties and these ingredients may be helpful for developing designer functional foods with cancer-preventive properties. |
Mendeley readers
The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 1 | 1% |
| United States | 1 | 1% |
| Poland | 1 | 1% |
| Argentina | 1 | 1% |
| Unknown | 63 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 18% |
| Student > Ph. D. Student | 11 | 16% |
| Student > Master | 9 | 13% |
| Professor | 8 | 12% |
| Student > Doctoral Student | 6 | 9% |
| Other | 9 | 13% |
| Unknown | 12 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 28 | 42% |
| Chemistry | 7 | 10% |
| Medicine and Dentistry | 5 | 7% |
| Biochemistry, Genetics and Molecular Biology | 3 | 4% |
| Engineering | 3 | 4% |
| Other | 6 | 9% |
| Unknown | 15 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 February 2013.
All research outputs
#7,535,755
of 22,992,311 outputs
Outputs from Breast Cancer Research and Treatment
#1,674
of 4,676 outputs
Outputs of similar age
#19,017
of 58,533 outputs
Outputs of similar age from Breast Cancer Research and Treatment
#8
of 17 outputs
Altmetric has tracked 22,992,311 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,676 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.2. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 58,533 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one is in the 5th percentile – i.e., 5% of its contemporaries scored the same or lower than it.