| Title |
Membrane distribution of the glycine receptor α3 studied by optical super-resolution microscopy
|
|---|---|
| Published in |
Histochemistry and Cell Biology, February 2014
|
| DOI | 10.1007/s00418-014-1197-y |
| Pubmed ID | |
| Authors |
Kristof Notelaers, Susana Rocha, Rik Paesen, Nina Swinnen, Jeroen Vangindertael, Jochen C. Meier, Jean-Michel Rigo, Marcel Ameloot, Johan Hofkens |
| Abstract |
In this study, the effect of glycine receptor (GlyR) α3 alternative RNA splicing on the distribution of receptors in the membrane of human embryonic kidney 293 cells is investigated using optical super-resolution microscopy. Direct stochastic optical reconstruction microscopy is used to image both α3K and α3L splice variants individually and together using single- and dual-color imaging. Pair correlation analysis is used to extract quantitative measures from the resulting images. Autocorrelation analysis of the individually expressed variants reveals clustering of both variants, yet with differing properties. The cluster size is increased for α3L compared to α3K (mean radius 92 ± 4 and 56 ± 3 nm, respectively), yet an even bigger difference is found in the cluster density (9,870 ± 1,433 and 1,747 ± 200 μm(-2), respectively). Furthermore, cross-correlation analysis revealed that upon co-expression, clusters colocalize on the same spatial scales as for individually expressed receptors (mean co-cluster radius 94 ± 6 nm). These results demonstrate that RNA splicing determines GlyR α3 membrane distribution, which has consequences for neuronal GlyR physiology and function. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Hungary | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 2 | 5% |
| Argentina | 1 | 3% |
| Belgium | 1 | 3% |
| Unknown | 33 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 9 | 24% |
| Researcher | 7 | 19% |
| Student > Ph. D. Student | 6 | 16% |
| Student > Postgraduate | 4 | 11% |
| Student > Bachelor | 3 | 8% |
| Other | 6 | 16% |
| Unknown | 2 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 30% |
| Biochemistry, Genetics and Molecular Biology | 8 | 22% |
| Physics and Astronomy | 5 | 14% |
| Chemistry | 3 | 8% |
| Medicine and Dentistry | 2 | 5% |
| Other | 5 | 14% |
| Unknown | 3 | 8% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 February 2014.
All research outputs
#19,702,729
of 24,217,893 outputs
Outputs from Histochemistry and Cell Biology
#681
of 926 outputs
Outputs of similar age
#168,996
of 229,018 outputs
Outputs of similar age from Histochemistry and Cell Biology
#9
of 13 outputs
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