| Title |
Quantitative single-molecule localization microscopy combined with rule-based modeling reveals ligand-induced TNF-R1 reorganization toward higher-order oligomers
|
|---|---|
| Published in |
Histochemistry and Cell Biology, February 2014
|
| DOI | 10.1007/s00418-014-1195-0 |
| Pubmed ID | |
| Authors |
Franziska Fricke, Sebastian Malkusch, Gaby Wangorsch, Johannes F. Greiner, Barbara Kaltschmidt, Christian Kaltschmidt, Darius Widera, Thomas Dandekar, Mike Heilemann |
| Abstract |
We report on the assembly of tumor necrosis factor receptor 1 (TNF-R1) prior to ligand activation and its ligand-induced reorganization at the cell membrane. We apply single-molecule localization microscopy to obtain quantitative information on receptor cluster sizes and copy numbers. Our data suggest a dimeric pre-assembly of TNF-R1, as well as receptor reorganization toward higher oligomeric states with stable populations comprising three to six TNF-R1. Our experimental results directly serve as input parameters for computational modeling of the ligand-receptor interaction. Simulations corroborate the experimental finding of higher-order oligomeric states. This work is a first demonstration how quantitative, super-resolution and advanced microscopy can be used for systems biology approaches at the single-molecule and single-cell level. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Hungary | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 72 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 1% |
| Netherlands | 1 | 1% |
| France | 1 | 1% |
| United Kingdom | 1 | 1% |
| Argentina | 1 | 1% |
| Unknown | 67 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 18 | 25% |
| Student > Ph. D. Student | 15 | 21% |
| Student > Master | 8 | 11% |
| Student > Bachelor | 6 | 8% |
| Student > Postgraduate | 6 | 8% |
| Other | 12 | 17% |
| Unknown | 7 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 26 | 36% |
| Biochemistry, Genetics and Molecular Biology | 12 | 17% |
| Physics and Astronomy | 10 | 14% |
| Chemistry | 8 | 11% |
| Medicine and Dentistry | 3 | 4% |
| Other | 7 | 10% |
| Unknown | 6 | 8% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 February 2014.
All research outputs
#19,702,729
of 24,217,893 outputs
Outputs from Histochemistry and Cell Biology
#681
of 926 outputs
Outputs of similar age
#243,453
of 322,394 outputs
Outputs of similar age from Histochemistry and Cell Biology
#9
of 13 outputs
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