| Title |
High prevalence of subtype 4 among isolates of Blastocystis hominis from symptomatic patients of a health district of Valencia (Spain)
|
|---|---|
| Published in |
Parasitology Research, May 2009
|
| DOI | 10.1007/s00436-009-1485-y |
| Pubmed ID | |
| Authors |
M. Victoria Domínguez-Márquez, Remedios Guna, Carlos Muñoz, M. Teresa Gómez-Muñoz, Rafael Borrás |
| Abstract |
In order to know the genetic diversity of Blastocystis hominis from a health district of Valencia (Spain) 51 clinical isolates from symptomatic patients, 31 axenic and 20 monoxenic, were ribotyped by analysing the restriction fragment length polymorphism (RFLP) of amplicons obtained by polymerase chain reaction (PCR) of small-subunit of ribosomal DNA genes (SSU-rDNA). For this purpose, DNA was subjected to two independent PCR (RD3-RD5, F1-R1) and to three independent treatments with restrictases (AluI, HinfI and RsaI). The digested DNA was separated electrophoretically, the isolates were clustered into ribotypes (ribodemes, RD3-RD5; subgroups, F1-R1) according to their profiles and the results were translated into genetic subtypes (ST) proposed by a consensus terminology. The results show that the isolates studied are an heterogeneous population and that both PCR-RFLP SSU-rDNA protocols have a similar discriminative power, since it allowed the ribotyping of all isolates and their clustering into four demes: ribodemes 1, 3 and 3-r and 6, which include isolates belonging to subgroup III, IV, V and V-r, respectively; which were assigned to ST1 (2%), ST2 (3.9%) and ST4 (94.1%). The most common of which is a zoonotic subtype (Blastocystis ratti) which includes, according to recent studies, non-pathogenic and pathogenic variants. |
Mendeley readers
The data shown below were compiled from readership statistics for 72 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 72 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 11 | 15% |
| Student > Master | 9 | 13% |
| Student > Bachelor | 9 | 13% |
| Student > Doctoral Student | 8 | 11% |
| Student > Ph. D. Student | 8 | 11% |
| Other | 12 | 17% |
| Unknown | 15 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 15 | 21% |
| Immunology and Microbiology | 13 | 18% |
| Biochemistry, Genetics and Molecular Biology | 10 | 14% |
| Agricultural and Biological Sciences | 9 | 13% |
| Veterinary Science and Veterinary Medicine | 3 | 4% |
| Other | 8 | 11% |
| Unknown | 14 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 September 2012.
All research outputs
#7,535,755
of 22,992,311 outputs
Outputs from Parasitology Research
#626
of 3,799 outputs
Outputs of similar age
#38,056
of 112,501 outputs
Outputs of similar age from Parasitology Research
#8
of 21 outputs
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