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Post‐crystallization treatments for improving diffraction quality of protein crystals

Overview of attention for article published in Acta Crystallographica: Section D (International Union of Crystallography - IUCr), August 2005
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Title
Post‐crystallization treatments for improving diffraction quality of protein crystals
Published in
Acta Crystallographica: Section D (International Union of Crystallography - IUCr), August 2005
DOI 10.1107/s0907444905019451
Pubmed ID
Authors

Begoña Heras, Jennifer L. Martin

Abstract

X-ray crystallography is the most powerful method for determining the three-dimensional structure of biological macromolecules. One of the major obstacles in the process is the production of high-quality crystals for structure determination. All too often, crystals are produced that are of poor quality and are unsuitable for diffraction studies. This review provides a compilation of post-crystallization methods that can convert poorly diffracting crystals into data-quality crystals. Protocols for annealing, dehydration, soaking and cross-linking are outlined and examples of some spectacular changes in crystal quality are provided. The protocols are easily incorporated into the structure-determination pipeline and a practical guide is provided that shows how and when to use the different post-crystallization treatments for improving crystal quality.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 512 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 6 1%
Germany 4 <1%
Russia 3 <1%
Japan 3 <1%
France 2 <1%
United States 2 <1%
India 1 <1%
Czechia 1 <1%
Sweden 1 <1%
Other 8 2%
Unknown 481 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 147 29%
Researcher 139 27%
Student > Master 48 9%
Student > Bachelor 26 5%
Student > Doctoral Student 24 5%
Other 76 15%
Unknown 52 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 247 48%
Biochemistry, Genetics and Molecular Biology 134 26%
Chemistry 43 8%
Physics and Astronomy 6 1%
Medicine and Dentistry 6 1%
Other 19 4%
Unknown 57 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 February 2014.
All research outputs
#17,285,668
of 25,374,647 outputs
Outputs from Acta Crystallographica: Section D (International Union of Crystallography - IUCr)
#1,904
of 2,783 outputs
Outputs of similar age
#52,570
of 59,074 outputs
Outputs of similar age from Acta Crystallographica: Section D (International Union of Crystallography - IUCr)
#19
of 20 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,783 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 59,074 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 5th percentile – i.e., 5% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.