| Title |
In situ monitoring of molecular changes during cell differentiation processes in marine macroalgae through mass spectrometric imaging
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, June 2017
|
| DOI | 10.1007/s00216-017-0430-7 |
| Pubmed ID | |
| Authors |
Ralf W. Kessler, Anna C. Crecelius, Ulrich S. Schubert, Thomas Wichard |
| Abstract |
Matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) was employed to discriminate between cell differentiation processes in macroalgae. One of the key developmental processes in the algal life cycle is the production of germ cells (gametes and zoids). The gametogenesis of the marine green macroalga Ulva mutabilis (Chlorophyta) was monitored by metabolomic snapshots of the surface, when blade cells differentiate synchronously into gametangia and giving rise to gametes. To establish MSI for macroalgae, dimethylsulfoniopropionate (DMSP), a known algal osmolyte, was determined. MSI of the surface of U. mutabilis followed by chemometric data analysis revealed dynamic metabolomic changes during cell differentiation. DMSP and a total of 55 specific molecular biomarkers, which could be assigned to important stages of the gametogenesis, were detected. Our research contributes to the understanding of molecular mechanisms underlying macroalgal cell differentiation. Graphical abstract Molecular changes during cell differentiation of the marine macroalga Ulva were visualized by matrix assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI). |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 37 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 7 | 19% |
| Researcher | 5 | 14% |
| Student > Ph. D. Student | 4 | 11% |
| Other | 2 | 5% |
| Student > Doctoral Student | 2 | 5% |
| Other | 6 | 16% |
| Unknown | 11 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 32% |
| Chemistry | 4 | 11% |
| Biochemistry, Genetics and Molecular Biology | 2 | 5% |
| Unspecified | 2 | 5% |
| Medicine and Dentistry | 2 | 5% |
| Other | 4 | 11% |
| Unknown | 11 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 July 2017.
All research outputs
#20,660,571
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Outputs from Analytical & Bioanalytical Chemistry
#6,602
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Outputs of similar age
#255,115
of 331,653 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#88
of 148 outputs
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