| Title |
Na+/H+ exchanger 3 inhibitor diminishes hepcidin-enhanced duodenal calcium transport in hemizygous β-globin knockout thalassemic mice
|
|---|---|
| Published in |
Molecular and Cellular Biochemistry, December 2016
|
| DOI | 10.1007/s11010-016-2911-y |
| Pubmed ID | |
| Authors |
Narattaphol Charoenphandhu, Kamonshanok Kraidith, Kornkamon Lertsuwan, Chanakarn Sripong, Panan Suntornsaratoon, Saovaros Svasti, Nateetip Krishnamra, Kannikar Wongdee |
| Abstract |
Recent investigation has shown that the liver-derived iron-regulating hormone, hepcidin, can potentiate intestinal calcium absorption in hemizygous β-globin knockout thalassemic (BKO) mice. Since the upregulation of Fe(2+) and H(+) cotransporter, divalent metal transporter (DMT)-1, has been shown to correlate with thalassemia-induced intestinal calcium absorption impairment, the inhibition of the apical Na(+)/H(+) exchanger (NHE)-3 that is essential for cytoplasmic pH regulation and transepithelial sodium absorption was hypothesized to negatively affect hepcidin action. Herein, the positive effect of hepcidin on the duodenal calcium transport was evaluated using Ussing chamber technique. The results showed that BKO mice had lower absorptive surface area and duodenal calcium transport than wild-type mice. Besides, paracellular transport of zinc in BKO mice was compromised. Hepcidin administration completely restored calcium transport. Since this hepcidin action was totally abolished by inhibitors of the basolateral calcium transporters, Na(+)/Ca(2+) exchanger (NCX1) and plasma membrane Ca(2+)-ATPase (PMCA1b), the enhanced calcium flux potentially occurred through the transcellular pathway rather than paracellular pathway. Interestingly, the selective NHE3 inhibitor, 100 nM tenapanor, markedly inhibited hepcidin-enhanced calcium transport. Accordingly, hepcidin is one of the promising therapeutic agents for calcium malabsorption in β-thalassemia. It mainly stimulates the transcellular calcium transport across the duodenal epithelium in an NHE3-dependent manner. |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 28 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 3 | 11% |
| Student > Ph. D. Student | 2 | 7% |
| Student > Doctoral Student | 2 | 7% |
| Other | 1 | 4% |
| Student > Master | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 18 | 64% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 6 | 21% |
| Biochemistry, Genetics and Molecular Biology | 1 | 4% |
| Veterinary Science and Veterinary Medicine | 1 | 4% |
| Immunology and Microbiology | 1 | 4% |
| Neuroscience | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 18 | 64% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 June 2019.
All research outputs
#4,726,883
of 22,992,311 outputs
Outputs from Molecular and Cellular Biochemistry
#194
of 2,319 outputs
Outputs of similar age
#95,409
of 421,064 outputs
Outputs of similar age from Molecular and Cellular Biochemistry
#1
of 21 outputs
Altmetric has tracked 22,992,311 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,319 research outputs from this source. They receive a mean Attention Score of 3.9. This one has done particularly well, scoring higher than 91% of its peers.
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We're also able to compare this research output to 21 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.