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A. phagocytophilum invasin AipA

Overview of attention for article published in Cellular Microbiology, April 2014
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#8 of 1,673)
  • High Attention Score compared to outputs of the same age (96th percentile)
  • High Attention Score compared to outputs of the same age and source (94th percentile)

Mentioned by

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5 news outlets
blogs
1 blog
twitter
2 X users
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2 patents
facebook
1 Facebook page

Citations

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27 Dimensions

Readers on

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27 Mendeley
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Title
A. phagocytophilum invasin AipA
Published in
Cellular Microbiology, April 2014
DOI 10.1111/cmi.12286
Pubmed ID
Authors

David Seidman, Nore Ojogun, Naomi J. Walker, Juliana Mastronunzio, Amandeep Kahlon, Kathryn S. Hebert, Sophia Karandashova, Daniel P. Miller, Brittney K. Tegels, Richard T. Marconi, Erol Fikrig, Dori L. Borjesson, Jason A. Carlyon

Abstract

Anaplasma phagocytophilum, which causes granulocytic anaplasmosis in humans and animals, is a tick-transmitted obligate intracellular bacterium that mediates its own uptake into neutrophils and non-phagocytic cells. Invasins of obligate intracellular pathogens are attractive targets for protecting against or curing infection because blocking the internalization step prevents survival of these organisms. The complement of A. phagocytophilum invasins is incompletely defined. Here, we report the significance of a novel A. phagocytophilum invasion protein, AipA. A. phagocytophilum induced aipA expression during transmission feeding of infected ticks on mice. The bacterium upregulated aipA transcription when it transitioned from its non-infectious reticulate cell morphotype to its infectious dense-cored morphotype during infection of HL-60 cells. AipA localized to the bacterial surface and was expressed during in vivo infection. Of the AipA regions predicted to be surface-exposed, only residues 1 to 87 (AipA1-87 ) were found to be essential for host cell invasion. Recombinant AipA1-87 protein bound to and competitively inhibited A. phagocytophilum infection of mammalian cells. Antiserum specific for AipA1-87 , but not other AipA regions, antagonized infection. Additional blocking experiments using peptide-specific antisera narrowed down the AipA invasion domain to residues 9 to 21. An antisera combination targeting AipA1-87 together with two other A. phagocytophilum invasins, OmpA and Asp14, nearly abolished infection of host cells. This study identifies AipA as an A. phagocytophilum surface protein that is critical for infection, demarcates its invasion domain, and establishes a rationale for targeting multiple invasins to protect against granulocytic anaplasmosis.

X Demographics

X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Czechia 1 4%
Brazil 1 4%
Unknown 25 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 30%
Researcher 4 15%
Student > Bachelor 2 7%
Professor > Associate Professor 2 7%
Student > Master 2 7%
Other 5 19%
Unknown 4 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 33%
Biochemistry, Genetics and Molecular Biology 5 19%
Veterinary Science and Veterinary Medicine 4 15%
Medicine and Dentistry 2 7%
Immunology and Microbiology 1 4%
Other 0 0%
Unknown 6 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 48. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 January 2022.
All research outputs
#874,091
of 25,374,917 outputs
Outputs from Cellular Microbiology
#8
of 1,673 outputs
Outputs of similar age
#8,284
of 238,627 outputs
Outputs of similar age from Cellular Microbiology
#1
of 17 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,673 research outputs from this source. They receive a mean Attention Score of 4.4. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 238,627 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 96% of its contemporaries.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.