The purpose of this study was to investigate the relationship between overall physical workload (GPS/accelerometer) measures and injury risk in elite Australian football players (n=46) during a season. Workload data and (intrinsic) injury incidence was monitored across pre-season and in-season (18 matches) phases. Multiple regression was used to compare cumulative (1-, 2-, 3-, 4-weekly loads) and absolute change (from previous-to-current week) in workloads between injured and uninjured players for all GPS/accelerometer-derived variables: total distance; V1 distance (total distance above individual's aerobic threshold speed); sprint distance; force load; velocity load and relative velocity change. Odds ratios (OR) were calculated to determine the relative injury risk. Cumulative loads showed the strongest relationship with greater intrinsic injury risk. During pre-season, 3-weekly distance (OR=5.489, p=0.008) and 3-weekly sprint distance (OR=3.667, p=0.074) were most indicative of greater injury risk. In-season, 3-weekly force load (OR=2.530, p=0.031) and 4-weekly relative velocity change (OR= 2.244, p=0.035) were associated with greater injury risk. No differences in injury risk between years of AFL system experience and GPS/accelerometer data were seen. From an injury risk (prevention) perspective, these findings support consideration of several GPS/accelerometer running load variables in Australian football players. In particular, cumulative weekly loads should be closely monitored, with 3-weekly loads most indicative of a greater injury risk across both seasonal phases. (224 words).