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X Demographics
Mendeley readers
Attention Score in Context
| Title |
High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells
|
|---|---|
| Published in |
Nature, April 2014
|
| DOI | 10.1038/nature13166 |
| Pubmed ID | |
| Authors |
Yuexin Zhou, Shiyou Zhu, Changzu Cai, Pengfei Yuan, Chunmei Li, Yanyi Huang, Wensheng Wei |
| Abstract |
Targeted genome editing technologies are powerful tools for studying biology and disease, and have a broad range of research applications. In contrast to the rapid development of toolkits to manipulate individual genes, large-scale screening methods based on the complete loss of gene expression are only now beginning to be developed. Here we report the development of a focused CRISPR/Cas-based (clustered regularly interspaced short palindromic repeats/CRISPR-associated) lentiviral library in human cells and a method of gene identification based on functional screening and high-throughput sequencing analysis. Using knockout library screens, we successfully identified the host genes essential for the intoxication of cells by anthrax and diphtheria toxins, which were confirmed by functional validation. The broad application of this powerful genetic screening strategy will not only facilitate the rapid identification of genes important for bacterial toxicity but will also enable the discovery of genes that participate in other biological processes. |
X Demographics
The data shown below were collected from the profiles of 23 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | 13% |
| United States | 2 | 9% |
| Ireland | 1 | 4% |
| Switzerland | 1 | 4% |
| Denmark | 1 | 4% |
| Israel | 1 | 4% |
| China | 1 | 4% |
| India | 1 | 4% |
| Unknown | 12 | 52% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 17 | 74% |
| Scientists | 5 | 22% |
| Science communicators (journalists, bloggers, editors) | 1 | 4% |
Mendeley readers
The data shown below were compiled from readership statistics for 1,787 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 23 | 1% |
| United Kingdom | 12 | <1% |
| Germany | 6 | <1% |
| China | 5 | <1% |
| Japan | 5 | <1% |
| France | 4 | <1% |
| Spain | 4 | <1% |
| Austria | 3 | <1% |
| Brazil | 2 | <1% |
| Other | 14 | <1% |
| Unknown | 1709 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 465 | 26% |
| Researcher | 402 | 22% |
| Student > Master | 184 | 10% |
| Student > Bachelor | 159 | 9% |
| Student > Doctoral Student | 87 | 5% |
| Other | 261 | 15% |
| Unknown | 229 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 736 | 41% |
| Biochemistry, Genetics and Molecular Biology | 483 | 27% |
| Medicine and Dentistry | 83 | 5% |
| Immunology and Microbiology | 48 | 3% |
| Engineering | 38 | 2% |
| Other | 140 | 8% |
| Unknown | 259 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 34. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 February 2024.
All research outputs
#1,211,976
of 25,931,626 outputs
Outputs from Nature
#35,039
of 99,122 outputs
Outputs of similar age
#11,512
of 242,396 outputs
Outputs of similar age from Nature
#521
of 1,002 outputs
Altmetric has tracked 25,931,626 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 95th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 99,122 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 102.9. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 242,396 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 1,002 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.